Oral Administration of Vitamin C, Cimetidine and Famotidine on Micronuclei Induced by Low Dose Radiationin in Mouse Bone Marrow Cells
Journal Title: Journal of Biomedical Physics and Engineering - Year 2017, Vol 7, Issue 2
Abstract
Background: In many studies, chemicals and natural materials were tested to reduce the harmful effects of radiation. It is known that Famotidine and vitamin C reduce DNA damage. Objective: The aim of this study was to evaluate the radioprotective effect of vitamin C, Cimetidine and Famotidine on gamma-radiation-induced damage on mouse bone marrow. Methods: six-to-seven week male NMRI mice (28 g ±3) were randomly divided into fourteen groups: control, 2Gy irradiation, six group drugs without irradiation (Famotidine, Cimetidine, vitaminC, Fam-Cim, Fam-Vit, Cim-Vit), six groups received drugs and 2Gy radiation with a 60Co |γ|-ray source at the doses of 2 at room temperature 22 ± 2 °C. The mice 48 hours after radiation were killed by cervical dislocation, after staining with Gimsa-May Granvaldsmire form femur bone marrow was prepared. Finally, the cells were counted with microscope, frequencies of pollychoromaticerythrocyte (PCE), normochoromatic (NCE) and their micronuclated cell were counted. PCE / PCE + NCE were calculated. Results: There were significant differences of MNPCE/1000PCE, MNNCE/1000NCE and PCE/PCE+NCE among different groups with similar radiation doses (p≤0.01). Moreover, there were significant differences of MNPCE/1000PCE and PCE/PCE+NCE among different doses of radiation (p≤0.01). While considering MNNCE/1000NCE, there were no significant differences among silimar groups with radiation dose (p˃0.05). Conclusion: Oral administration of Famotidine, vitamin C and Cimetidine demonstrate reliable and similar radioprotective effects. Additionally, the protective effect of single use of these drugs was similar to the combination form. Thus, the oral use of combination, 48 hours after irradiation cannot induce more radioprotective effect.
Authors and Affiliations
A Naeeji, H Mozdarani, F Faeghi, A A Ahmadi, M Gholami, R Behzadi, M R Momtaz
Keywords
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