Oxidation of anthracene by phthalic anhydride modified laccase
Journal Title: Science Paper Online - Year 2007, Vol 2, Issue 12
Abstract
Laccase is modified with phthalic anhydride in order to improve its stability against high temperature and acidic pH in this paper. The modification ratio of amino lysine groups of laccase is determined as 63.8% using TNBS method. It is shown that the modified laccase maintained the secondary and tertiary structure of its native counterpart but has a higher affinity to ABTS and an significantly improved thermal stability, as indicated by the extension of the half-life of enzyme activity at 55C from 192.5 min to 532.4 min. The suitable pH range is also expended from pH5.9~7.8 to pH4.5~8.4. The degradation ratio of anthracene is increased by 2-fold, making the modified laccase promising for the removal of PAHs.
Authors and Affiliations
Xiufu HUA , Jun GE, Su LI
Keywords
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