Preparation and application of multicolor luminescent lanthanide nanoparticles
Journal Title: Science Paper Online - Year 2009, Vol 4, Issue 9
Abstract
Because strongly fluorescent lanthanide compounds are mainly limited to Eu3+ and Tb3+ compounds with red (Eu, ~615 nm) and green (Tb, ~545 nm) emissions, the development and application of fluorescence bioassay technique using lanthanide-based multicolor fluorescent biolabels are rarely investigated. A series of lanthanide complexes-bound silica nanoparticles with red, orange, yellow and green fluorescence colors was prepared by co-binding different ratios of Eu3+/Tb3+ fluorescent complexes with the ligand (4’-phenyl-2,2’:6’,2”-terpyridine-6,6”-diyl) bis(methylenenitrilo) tetrakis (acetic acid) inside the silica nanoparticles. The nanoparticle-labeled streptavidin was prepared and used for time-resolved fluorescence detections of human prostate-specific antigen and an environmental pathogen Giardia lamblia. The results indicate that the new nanoparticles are useful to multicolor biolabels for time-resolved fluorescence bioassays.
Authors and Affiliations
Hongfei Jiang, Zhiqiang Ye, Xiaoyu Liu
Keywords
Related Articles
- EP ID EP113212
- DOI -
- Views 109
- Downloads 0
Pt与Fe的加入对NSR催化剂Ba/Al2O3<br /> 结构和性能的影响<br />
本文通过制备一系列Ba/Al2O3催化剂，探讨进一步加入Pt、Fe对催化剂结构、NOx储存能力及耐硫中毒能力的影响。结果表明：Ba/Al2O3催化剂存在着两种不同的Ba物种，即与载体紧密接触的高分散的Ba物种以及体相BaAl2O4尖晶石相。Fe的加入抑制Ba...
Disscussion on deductive preference and non-deductive preference <br /> in mathematical proof<br /> ——The answer for some questions<br />
Non-deductive preference may improve the understanding of the mathematical proof and the preference in it. Non-deductive preference is feasible in natural and social science owing to the highest abstraction and generaliz...
不同掺杂二氧化钛的电子和晶体结构与<br /> 光催化性能的关系<br />
对未掺杂、氮掺杂、碘掺杂和铂掺杂的锐钛矿TiO2进行了第一性原理计算。结果表明，三种掺杂TiO2带隙中都产生新的能带。这些新出现的能带是掺杂TiO2响应可见光的原因。而且铂掺杂TiO2的带隙缩小，响应波长更宽。铂掺杂和碘TiO2的能带位置明...
软骨多糖对L1210细胞增殖抑制作用的研究
建立DBA/2小鼠LI210腹水瘤模型，将小鼠分为5组进行实验，通过腹腔注射软骨多糖治疗，每天称重并记录小鼠的生存时间，计算生命延长率。于0 h、24 h、48 h、72 h抽取治疗组小鼠的腹水瘤细胞进行吉姆萨(Giemsa)染色观察细胞形态学变化；应用...
The present status and the key issues to be resolved of thick seam mining technique in China
This paper introduces the state of the art and the development of thick seam mining techniques in details and the prominent achievements in top-coal caving and large cutting height workface in China. It is pointed out th...