Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats
Journal Title: Sultan Qaboos University Medical Journal - Year 2014, Vol 14, Issue 3
Abstract
Objectives: Induced dilated cardiomyopathy is the main limitation of the anti-cancer drug doxorubicin, which causes oxidative stress and cardiomyocyte death. As ozone therapy can activate the antioxidant systems, this study aimed to investigate the therapeutic efcacy of ozone-oxidative preconditioning against doxorubicin-induced cardiotoxicity. Methods: Te study was carried out from September 2013 to January 2014. Sprague-Dawley rats were randomly distributed in the following treatment groups: Group 1 were treated with 2 mg/kg intraperitoneal (i.p.) of doxorubicin twice a week for 50 days; Group 2 were treated with 0.3 mg of ozone/oxygen mixture at 50 μg/ mL of ozone per 6 mL of oxygen by rectal insufation and then treated with doxorubicin; Group 3 were treated as Group 2 but only with the oxygen, and Group 4 were treated with oxygen frst, and then with sodium chloride i.p. as the control group. Results: Te results showed that ozone therapy preserved left ventricle morphology which was accompanied by a reduction of serum pro-brain natriuretic peptide levels. Te cardioprotective effects of ozoneoxidative preconditioning were associated with a signifcant increase (P <0.05) of antioxidant enzymes activities and a reduction of lipid and protein oxidation (P <0.05). Conclusion: Ozone-oxidative preconditioning prevents doxorubicin-induced dilated cardiomyopathy through an increase of antioxidant enzymes and a reduction of oxidised macromolecules. Tis establishes the background for future studies to determine if ozone therapy can be used as a complementary treatment for attenuating doxorubicin-induced cardiotoxicity in cancer patients.
Authors and Affiliations
Livan Delgado-Roche| Center of Studies for Research & Biological Evaluations, Pharmacy & Food Science College, University of Havana, Cuba, Yanet Hernández-Matos| Center of Studies for Research & Biological Evaluations, Pharmacy & Food Science College, University of Havana, Cuba, Emilio A. Medina| Center of Studies for Research & Biological Evaluations, Pharmacy & Food Science College, University of Havana, Cuba, Dalia Á. Morejón| Center of Studies for Research & Biological Evaluations, Pharmacy & Food Science College, University of Havana, Cuba, Maité R. González| LABIOFAM Pharmaceutical Laboratories, Havana, Cuba, Gregorio Martίnez-Sánchez| Medical Center Beauty Beneft, Osimo, Ancona, Italy
Keywords
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