Papillomavirus: Viral vectors in the gene therapy and new therapeutic targets
Journal Title: International Journal of Biomedical Research - Year 2015, Vol 6, Issue 10
Abstract
Epitheliotropic and mucosotropic Papillomaviruses (PVs) constitute the Papillomaviridae family. There are 26 genera comprising levels of inter species genetic similarities by nucleotide sequence analysis of the L1 viral region, the generally most conserved region among papillomaviruses. There is an increasing consensus that the ideal vector for gene therapy should be completely based on chromosomal elements and behave as an independent functional unit after integration. The present paper is a short review about epidemiology of HPV infection, vaccines anti-HPV and tumors associated with HPV and gene therapy including biological vectors, stem cells, viral and non-viral vectors and chromosome-based vectors. Furthermore, new therapeutic targets and vectors applied in gene therapy of other viruses are some of the examples that have been successfully modified by recombinant DNA techniques. New biotechnologies in molecular biology have been applied in papillomavirus research and are still being investigated for the application in the gene therapy. RNA-sequencing (RNA-seq) for analysis of the transcriptome, multiply-primed rolling circle amplification (RCA) used to identify novel human papillomaviruses (HPV) and new DNA sequencing method based on real-time phyrophosphate are examples as new techniques.
Authors and Affiliations
Rachel Siqueira de Queiroz Simoes, Ortrud Barth
Keywords
Related Articles
- EP ID EP419986
- DOI 10.7439/ijbr.v6i10.2555
- Views 87
- Downloads 0
Increase in catheter associated urinary tract infections in intensive care units at a tertiary care centre: a cause of concern
Aim: Health-care associated infections (HAIs) affect 5-20% of patients admitted in the hospitals. Catheter-associated urinary tract infections (CAUTI) are the commonest HAIs accounting for about 35-45% of these nosocomia...
Relooking at Nitrofurantoin as drug of choice for empiric treatment of community acquired uncomplicated urinary tract infections: An effective and economic treatment option
Introduction: Urinary tract infections (UTI) caused by multidrug resistant bacteria necessitate the use of broad spectrum expensive antibiotics, which enhance antibiotic pressure. It would be ideal to look at older less...
Role of cytotechnique in evaluation of non infectious vesiculobullous lesions of skin
im: To evaluate the efficacy and diagnostic accuracy of cytological techniques in evaluation of noninfectious vesiculobulloous lesions of skin. Materials and methods: Tzanck smear prepared from vesiculobullous lesions....
Assessment of Large Gut Obstruction in Rural setup
Introduction: Large bowel Obstruction is a widespread, emergent condition, which requires early identification, meticulous work up and prompt surgical intervention, wherever indicated. Materials and Methods: 25 consecuti...
A Rare case report: Granular cell tumor of the scalp
Granular cell tumor is a soft tissue neoplasm that originates in the nervous system and arises at virtually any body site, but is mainly found in the skin, oral cavity or digestive tract. Most are benign and reportedly m...