Partial Purification And Characterization Of Polyphenol Oxidase From Wild Pears (Pyrus communis)
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2013, Vol 4, Issue 10
Abstract
precipitation, dialysis and ion exchange chromatography. Of the substrates tested, pyrogallol was the better substrate for PPO with a Km value of 8.7 mM at pH 5 and catechol is better with Km value 4.7 mM at pH 7. The enzyme showed high activity over a broad pH range of 4 – 8 so the optimum pH for PPO activity was found to be 5 and 7. The optimal temperature for enzyme activity was found to be 45°C in presence of pyrogallol but 55°C with catechol as substrate at pH 7. Km value for wild pear PPO is calculated 9.5 and 4.7 mM for catechol and 8.7 and 5.1 mM for pyrogallol at pHs 5 and 7 respectively. As can be seen, affinity of PPOs for various substrates varies widely.The enzyme showed a broad activity over a broad pH and temperature range. The thermal inactivation studies showed that the enzyme is heat resistant. The enzyme showed the highest activity toward pyrogallol and no activity toward tyrosine. Of the inhibitors tested, the most potent inhibitors was sodium kojic acid.
Authors and Affiliations
Shahryar saeidian *| Assistan professor of Biochemistry, Department of Biology, Payame Noor University, Iran, saeedyan@pnu.ac.ir, Afsane khosravi| Steudent of Master Science of Azad University
Keywords
Related Articles
- EP ID EP5704
- DOI -
- Views 288
- Downloads 8
Investigating EFL Adult Learners? Behavioral and Emotional Attitudes
Learning a language is influenced by a variety of factors. One of the most important of which are the learner’s attitude toward the target language. Behavioral and emotional attitudes are two kinds of the attitudes. Also...
Effect of seed inoculation with bio-fertilizer on germination and early growth of corn
In order to study effect of seed inoculation with nitragin as bio-fertilizer at concentrations of 6, 7 and 8 cc and distilled water as control on germination and early growth of corn, a laboratory experiment was conducte...
Force Transducer and control Model for AFM Nano-manipulator
In most recent works, manipulation process based on nano robotic system has been widely investigated. In this article a 3D model in order to describe relation between AFM (Atomic Force Microscope) cantilever’s deformatio...
Studying Stability of Fluorographene with DFT Method Introduction
Graphene is the monolayer, honeycomb lattice of carbon atoms and has zero band gap. There are different methods for band gap creation in graphene. One of them is graphene fluorinated. We investigate fluorographene proper...
Modeling in order to Implementation of production balancing determination the best path to transport raw materials with the aim of improving for total expected cost
This article Based on case study in Sanaye Felezi Iran Co. in this research, introduce a model in order to the production balance with the aim of improving for total expected cost. In the first step of research, introduc...