Paternal Age Influences Human Sperm Chromatin Sensitivity to Ionizing Radiation
Journal Title: Chettinad Health City Medical Journal - Year 2018, Vol 7, Issue 3
Abstract
Objective : To understand the age related sensitivity of human and mouse sperm chromatin to radiation. Study design: This prospective study examined the comparative in vitro radiosensitivity of sperm DNA from normozoospermic human ejaculate (N= 47) and mouse epididymal spermatozoa (N= 40), from various age groups. Human ejaculates and mouse sperm suspension were exposed to 0 and 5 Gy,-radiation using 60Cobalt tele-therapy unit and sperm DNA damage was assessed by single cell gel electrophoresis (neutral comet assay). Results: The DNA fragmentation analysis of 5 Gy irradiated spermatozoa has revealed a significant increase in olive tail moment (OTM) in men above 40 years (P<0.05). In contrast, the OTM did not vary in mouse spermato- zoa in any of the irradiated groups. Conclusion: Though, the data has emerged from in vitro radiation exposure, from the clinical point of view, the study warns that men who are above 40 years are at higher risk of acquiring sperm DNA fragmentation after therapeutic and accidental radiation exposures.
Authors and Affiliations
Shubhashree U, Fiona D'Souza, Guruprasad K, Satish Kumar Adiga
Keywords
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