PATHOGEN IMPACT ON THE ACTIVITY DYNAMICS OF POTATO SUSPENSION CELLS EXTRA-CELLULAR PEROXIDASE
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2005, Vol 1, Issue 1
Abstract
Changes in the activity of extracellular peroxidases were measured in cell suspension cultures of potato infected by Clavibacter michiganensis subsp. sepedonicus (Spieck. et Kotth.) Skapt et Burkh. The total extracellular peroxidases activity of the resistant potato variety was higher than that of the sensitive variety both before and after infection. The enzyme of the resistant variety had a рН optimum of 6.2, while that of the sensitive variety was 5.4. Extracellular peroxidases of the sensitive potato variety were activated 10 minutes after infection, and displayed highest activity 1.5-2 hours later. In the resistant variety, peroxidase activity rose sharply in the first minutes of infection, and second peak of activity occurred 1.5-2 hours later. The increase of extracellular peroxidases activity of the sensitive potato variety under pathogenesis is connected with the change of genome expression and synthesis of proteins. The increase of enzyme activity of resistant potato variety in the first moments of infection is not related to proteins synthesis and is apparently conditioned by the change of kinetic parameters.
Authors and Affiliations
I Graskova, O Potapenko, V Voinikov
Keywords
Related Articles
- EP ID EP149680
- DOI -
- Views 88
- Downloads 0
Role of Nitric oxide in regulation of H[sub]2[/sub]O[sub]2[/sub] mediating tolerance of plants to abiotic stress: A synergistic signaling approach
The relationship between abiotic stress, nitric oxide (NO) and Hydrogen peroxide (H[sub]2[/sub]O[sub]2[/sub]) is a challenging one. It is now clear that H[sub]2[/sub]O[sub]2[/sub] and NO function as signaling molecules i...
Effect of chelating agents and metal ions on nickel bioavailability and chlorophyll fluorescence response in wheat- An approach for attenuation of Ni stress
The objectives of the study are to analyze the physiological changes, biochemical alterations and attenuation of nickel toxicity effects in wheat seedlings under combined applications of Ni ions, metal chelators (EDTA/Ci...
Ecological-biological Aspects of Stipa krylovii Roshev Adaptation at the Initial Stages of Ontogenesis
Xerophytic cereal [i]Stipa krylovii [/i]Roshev is interesting as a relic with extensive capabilities to adapt to severe climatic conditions of Eastern Zabaikal’ye, which allows it to occupy a vast areal. The species unde...
Evaluation of some upland cotton (Gossypium hirsutum L.) varieties for salinity tolerance screening
This investigation aimed to evaluate five upland cotton (Gossypium hirsutum L.) (Aleppo118, Aleppo33/1, Aleppo90, Raqqa5, and Deir-Ezzor22) varieties based on physiological indices and genetic variation using AFLP mar...
Effect of NaCl priming duration and concentration on germination behavior of Tunisian safflower
Priming is an effective technique that improves germination of several crop species. That’s why this study was carried in order to evaluate the effect of NaCl seed priming techniques on germination and early growth of sa...