Peg-based temperature sensitive nanoparticle synthesis and their use in protein adsorption
Journal Title: Archive of Biomedical Science and Engineering - Year 2018, Vol 4, Issue 1
Abstract
In this study, temperature sensitive polymeric nanoparticles were prepared by emulsifier free emulsion polymerization. N-isopropylacrylamide (NIPA) has been used as main monomer to give temperature sensitivity and poly(ethyleneglycol) ethyl ether methacrylate (PEG-EEM) has been added to recipes as comonomer to aid in particle formation. The effect of NIPA, PEG-EEM and crosslinker content on particle size has been investigated. Temperature sensitivity or lower critical solution temperature (LCST) of the nanoparticles has been followed by Zeta Sizer as change in particle size. In order to determine the protein adsorption capacity of nanoparticles, adsorption experiments have been investigated as a function of temperature and PEG-EEM amount. The results showed that the adsorption capacity of both NIPA and NIPA/PEG-EEM latexes was increased similarly with the increase in temperature. However, amount of BSA adsorbed on NIPA/PEG-EEM particles has been slightly higher than that of for NIPA alone. This study showed that synthesized NIPA/PEG-EEM nanoparticles would be a good candidate for the further studies on protein adsorption.
Authors and Affiliations
Utkan Guldem
Keywords
Related Articles
- EP ID EP545700
- DOI 10.17352/abse.000010
- Views 51
- Downloads 0
Microwave Irradiated and Conventional Synthesis, Antibacterial Activity Evaluation Studies of Tryptamine-Azole-Fluoroquinolone Conjugates
Tryptamine was converted to the corresponding 1,2,4-triazole, 1,3,4-oxadiazole, 5-oxo-1,3-thia(oxa)zolidine and 5-(4-chlorophenyl)-1,3-thia(oxa)zole derivatives via several steps. 1,3,4-oxadiazole and 1,2,4-triazoles wer...
Fabrication and Characterization of HER2 Cell Receptor-Targeted Indocyanine Green-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles
Introduction: The aim of this study is to fabricate and characterize human epidermal growth factor receptor 2 (HER2)-targeted indocyanine green (ICG)-loaded poly (lactic-co-glycolic acid (PLGA) nanoparticles (HIPNPs). Me...
Nanoderm Extracellular Matrix for Reconstructive Surgery Applications
Introduction: Bacterial cellulose (BC) can be used in wide area of applied scientific, especially for tissue regeneration and regenerative medicine, lately, bacterial cellulose mats are used in the treatment of skin cond...
Coronary Artery Disease Diagnosis Using Supervised Fuzzy C-Means with Differential Search Algorithm-based Generalized Minkowski Metrics
Introduction: Coronary Artery Disease (CAD), one of the leading causes of death, is narrowing the walls of the coronary arteries. Angiography is the most accurate but invasive and costly CAD diagnosis method associated w...
Myocardial Contrast and Stress Echocardiography: New Frontiers
The assessment of myocardial perfusion by echocardiography following intravenous injection of ultrasound contrast agents has proved to be feasible [1]. Low-mechanical index imaging techniques that cause minimal microbubb...