Abstract

Examination of urine leukocyte count usually use random urine samples and urine examination is performed while urine is still fresh because formed elements in urine begins to be disintegrated within 2 hours. The purpose of this study is to determine the effect of urine storage time on the number of leukocytes in urine checked immediately (0 hours) and being kept 30, 60, 90, 120, 150 and 180 minutes at room temperature. Types of Research is true experiment with investigating the result of a treatment, research conducted on four samples of urine of patients with diabetes mellitus who meet several criteria, which are blood glucose levels> 200 mg / dl and the amount of urine leukocyte > 30 cells / mm3, calculated using the Improved Neubauer . The result shows a decrease in the number of stored urine leukocytes 30 minutes = 10%, 60 min = 25%, 90 min = 32%, 120 minutes = 39%, 150 minutes = 43%, and 180 minutes = 51%. Values obtained by linear regression test p = 0.034 (<α = 0.05). There is a significant relationship between duration of storage of urine with urine leukocyte counts which is immediately checked (0 min) and stored 30, 60, 90, 120, 150 and 180 minutes at room temperature. Examination of urine leukocyte counts should be performed immediately or use the urine that is still in a fresh state.

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