Phenotypic and genotypic characterization of a marine bacterium isolated from Sundarbans, Bangladesh
Journal Title: Scientific Journal of Microbiology - Year 2015, Vol 4, Issue 7
Abstract
The mangrove ecosystem is a largely unexplored source for bacteria with potentiality to produce biologically active secondary metabolites. Consequently, we set our research study to isolate, characterize and screen a bacterium producing bioactive compounds from marine soil of largest mangrove forest of the world, Sundarbans, Bangladesh. A total number of 39 marine bacterial colonies were isolated and purified. Representative bioactive isolate, ANAM-5 was characterized using phenotypic and genotypic procedures since it exhihibited highest antibacterial activity against a series of test organisms. The 16S rDNA sequence of the strain ANAM-5 showed close similarity with only one bacterium Planctomyces brasiliensis, complete genome (99.64%). But the cultural, morphological, physiological and biochemical characteristics of the strain and P. brasiliensis were completely different. So, it may not be logical to assign the strain ANAM-5 to the genus Planctomyces. Thus, to confirm the taxonomic position of the strain and to have a correct solution from this taxonomic problem, further studies is needed in respect to the DNA relatedness studies, fatty acid profiling, DNA-DNA hybridization, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), metabolic finger profiling using BIOLOG, ribotyping, small subunit (SSU) sequences, cell wall composition and other characterizations.
Authors and Affiliations
A. K. Sarker| Department of Pharmacy, Pabna University of Science and Technology, Pabna-6600, Bangladesh., A. Haque| Department of Pharmacy, Pabna University of Science and Technology, Pabna-6600, Bangladesh., M. S. Islam| Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh., U. Haque| Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh., N. K. Kundo| Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh., A. S. Anisuzzaman| Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.Department of Hematology & Medical Oncology, Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA., U. Saha| Eastern Medical College and Hospital, Comilla, Bangladesh., A. Ul. Islam*| Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
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- EP ID EP1206
- DOI 10.14196/sjm.v4i7.1867
- Views 479
- Downloads 30
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