Phenotypic Expression of erm Gene Among Staphylococcus aureus
Journal Title: National Journal of Laboratory Medicine - Year 2016, Vol 5, Issue 2
Abstract
Introduction: The increasing frequency of MRSA infections and rapidly changing patterns in antimicrobial resistance, led to renewed interest in the use of macrolide lincosamide– streptogramin B (MLSB) antibiotics to treat such infections. Aim: To assess the prevalence of phenotypic expression of inducible resistance for clindamycin due to expression of erm genes, in clinical isolates of Methicillin resistant Staphylococcus aureus from various clinical samples using modified D test. Materials and Methods: A total of 101 Methicillin resistant Staphylococcus aureus (MRSA) strains were included for induction tests which utilize closely approximated erythromycin, clindamycin and azithromycin discs (modified D test); the flattening or indentation of the clindamycin zone of inhibition adjacent to the erythromycin and / or azithromycin disk indicates inducible MLSB (macrolide lincosamide– streptogramin B) resistance. Results: Among the 101 clinical isolates, 37 (36.63%) isolates shows D test positive using Erythromycin, Clindamycin and Azithromycin combination. Inducible resistance to clindamycin using azithromycin was found in 5.94% isolates which was the major achievement of the study, which will be confirmed by genotyping in future. Conclusion: D-test should be performed on all MRSA isolates, to avoid erroneous reporting resulting in treatment failure, a partial modification in the D test can be made using azithromycin as it was helpful in our study, must be confirmed using genotyping.
Authors and Affiliations
Dr. Gaurav Dalela, Dr. Atul Vijay, Dr. Manoj Joshi
Keywords
Related Articles
- EP ID EP378932
- DOI 10.7860/NJLM/2016/17390:2107
- Views 139
- Downloads 0
Sertaconazole Nitrate 2% Cream for the Treatment of Tinea pedis
Introduction: Tinea pedis is a superficial fungal infection of the skin of feet caused by dermatophytes and characterized by erythema, desquamation, itching, vesicles and pustules in the interdigital or subdigital area,...
Comparison of Methods of Biofilm Detection in Urinary Candida Isolates and Evaluating its Role in Persistent Candiduria
Introduction: Candiduria is a common finding in catheterised patients with increasing trend of non albicans Candida species. Biofilm formation on indwelling catheters leads to persistent colonisation ending up in infecti...
Immunoreactivity of p53 in Urothelial Carcinomas of the Urinary Bladder
Introduction: Urothelial Carcinomas (UC) are heterogenous disease with unpredictable outcome. Risk stratification plays an important role in the management as 70% tumours are Non-Muscle Invasive (NMI) at the time of diag...
Significance of Early Onset Peaks on High Performance Liquid Chromatography
Letter to Editor
Intestinal Amoebiasis and Risk Factors Associated with its Transmission Among Paediatric Patients Attending a Tertiary Health Care Facility in Northern India
ABSTRACT Introduction: The occurrence of Entamoeba histolytica as a human intestinal parasite is a serious problem especially in developing countries. Despite the high prevalence of amoebiasis in various parts of India,...