Phenotypic Expression of erm Gene Among Staphylococcus aureus
Journal Title: National Journal of Laboratory Medicine - Year 2016, Vol 5, Issue 2
Abstract
Introduction: The increasing frequency of MRSA infections and rapidly changing patterns in antimicrobial resistance, led to renewed interest in the use of macrolide lincosamide– streptogramin B (MLSB) antibiotics to treat such infections. Aim: To assess the prevalence of phenotypic expression of inducible resistance for clindamycin due to expression of erm genes, in clinical isolates of Methicillin resistant Staphylococcus aureus from various clinical samples using modified D test. Materials and Methods: A total of 101 Methicillin resistant Staphylococcus aureus (MRSA) strains were included for induction tests which utilize closely approximated erythromycin, clindamycin and azithromycin discs (modified D test); the flattening or indentation of the clindamycin zone of inhibition adjacent to the erythromycin and / or azithromycin disk indicates inducible MLSB (macrolide lincosamide– streptogramin B) resistance. Results: Among the 101 clinical isolates, 37 (36.63%) isolates shows D test positive using Erythromycin, Clindamycin and Azithromycin combination. Inducible resistance to clindamycin using azithromycin was found in 5.94% isolates which was the major achievement of the study, which will be confirmed by genotyping in future. Conclusion: D-test should be performed on all MRSA isolates, to avoid erroneous reporting resulting in treatment failure, a partial modification in the D test can be made using azithromycin as it was helpful in our study, must be confirmed using genotyping.
Authors and Affiliations
Dr. Gaurav Dalela, Dr. Atul Vijay, Dr. Manoj Joshi
Keywords
Related Articles
- EP ID EP378932
- DOI 10.7860/NJLM/2016/17390:2107
- Views 148
- Downloads 0
Laboratory and Etiological Profile of Febrile Thrombocytopenia Cases- A Cross Sectional Study
Introduction: Fever is a symptom which is caused by a variety of illnesses and it usually occurs in response to an infection or inflammation. Patients presenting with fever in tropical country like India usually have an...
Prevalence and Antibiotic Resistance Pattern of Clinical Isolates of Enterococci from Blood Samples in A Tertiary Care Hospital, Jamnagar, Gujarat, India
Introduction: In recent years, enterococci have become important nosocomial pathogens. Indiscriminate use of antibiotics, prolonged hospital stay, severity of illness and immunosuppression are responsible for nosocomial...
Apolipoprotein B / Apolipoprotein A-I Ratio a better Diagnostic Marker of Coronary Heart Disease than Conventional LDL/HDL Ratio
Introduction: Apolipoprotein B (Apo B) and apolipoprotein A-I (Apo-I) are cholesterol transporters playing an important role in lipid metabolism. Apo B and A-I represent the total atherogenic and non-atherogenic particle...
Distribution of ABO, Rhesus Blood Groups, Transfusion Transmitted Diseases and Adverse Donor Reactions in a Blood Bank of a Tertiary Care Hospital: A Five Year Study
Introduction: Study of the distribution of A, B, O blood group systems is essential for genetic studies, medico – legal issues and importantly for efficient delivery of blood bank services. Also important is the immunolo...
Intraoral Schwannoma - Diagnosed by Fine Needle Aspiration Cytology: Report of Two Cases
Schwannomas are benign nerve sheath derived tumours and frequently involve the head and neck region. Intraoral involvement is rare and is seen in 1% of cases. Predilection of the lesion intraorally seen more in tongue fo...