Phosphate Solubilization Activity of Bacterial Strains Isolated from Gangetic Plains of North Bihar
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 6
Abstract
Phosphorous is considered as one of the vital macronutrients for the development and biological growth of plant. A survey report suggested that 98% of North Indian alluvial soil is deficient in phosphate because it not available in the form of minerals and even after secondary reaction it do not convert products into available form of soil nutrients unlike potassium (illite mineral). There are several phosphate solubilizing microorganisms (PSM) which have the capability to covert insoluble phosphate of soil into soluble forms by synthesizing organic acids. The present study was conducted to isolate bacteria from the alluvial soil of Gangetic plains of North Bihar and to evaluate their phosphate solubilization potential. A total of 18 microbial strains were isolated out of which 11 were bacteria and 7 were fungi. The two bacterial isolates S1(v)23 and S2(v)12 were selected for further study. The physiological, biochemical and molecular screening of purified isolates S1(v)23 and S2(V)12 identified as two distinct strains of Gram +ve Bacillus bacteria Aneurinibacillus migulanus. For phosphate solubilizing efficiency both the isolates and non-inoculated were cultured on PVK agar medium containing tri- calcium phosphate (TCP) for 15 days(360 hours).The maximum solubilization index was observed 4.19 in S1(v)23 and 3.94 in S2(v)12 PVK agar media. On the basis of halo zone formation, the qualitative and quantitative confirmatory analysis was done on Pikovskaya’s-Bromo Phenol Blue agar (PVK-BPB) and PVK-BPB broth medium again for 15 days. The decrease in pH value ranged from 3.87 and 4.12 by S1(v)23 and by S2(v)12 from initial pH of 7.0 whereas control maintained its pH 7.0. The colour intensity (at O.D. 600) change of media taken as an indicator of phosphate solubilizing efficiency of bacteria. The O.D. of both the isolates S1(v)23 , S2(v)12 were 0.632 and 1.091 whereas the O.D. of control was 1.282.Phosphate solubilizing ability test of these two isolates also showed that they can solubilize TCP from 54.01 to 139.12 mg/l and 52.03 to 118.79 mg/l.
Authors and Affiliations
Mini Tiwari, Ashok Ghosh, Ghanshayam Kumar Satyapal, Amar Kumar
Keywords
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- EP ID EP415187
- DOI 10.9790/264X-0406010108.
- Views 109
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