Physico-chemical characterization, analytical method development and solubility studies for progesterone
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2017, Vol 3, Issue 2
Abstract
Progesterone is a well known natural contraceptive, a drug of BCS class II, which was used in early centuries to protect from the side effects of estrogens like abortion. For the preformulation studies, the solubility of progesterone in various oils, solvents, surfactants and co-surfactants was determined for further formulation development. The Progesterone has been evaluated for XRD, DSC, Partition coefficient, melting point and its solubility is also checked in different solvents. Physico-chemical characterization studies showed that progesterone has a melting point of 1270C. The solubility of drug progesterone was evaluated in methanol, and ethanol. The analytical method developed for the estimation of progesterone in methanol and ethanol showed maximum absorbance at λmax of 241 nm and 247 nm respectively at pH 7.4.Linearity studies indicated that estimation of progesterone between 1.00 μg /ml to 10.00 μg /ml was found to be linear with regression equation of y = 0.0575x + 0.0041; R² = 0.9985 with methanol and y = 0.0442x+0.0041; R² = 0.9968 with ethanol. On the basis of solubility studies two oils with highest solubility (Oleic acid and Myritol), two surfactants (Tween80 and Tween20) and three co-surfactants (Ethanol, Propanol and PEG400) were selected respectively for further formulation studies. The above analytical parameters indicated that the developed UV Spectrophotometric method for progesterone was simple, accurate, precise and reproducible.
Authors and Affiliations
Shekhar Sharma| School of Pharmacy, Lloyd Institute of Management & Technology, Greater Noida, U.P, India, Anupama Diwan| School of Pharmaceutical Sciences, Apeejay Stya University, Gurgaon, Haryana, India, Rupali Kalra| School of Pharmaceutical Sciences, Apeejay Stya University, Gurgaon, Haryana, India, Vandana Arora| School of Pharmacy, Lloyd Institute of Management & Technology, Greater Noida, U.P, India
Keywords
Related Articles
- EP ID EP15588
- DOI -
- Views 367
- Downloads 21
Formulation and Evaluation of sustained release matrix tablets of Glipizide
The aim of present investigation was to enhance the solubility of glipizide (BCS Class II). Glipizide is an oral antidiabetic agent with relatively short elimination half life. Inclusion complex of Glipizide with -cyc...
A overview: non-steroidal anti-inflammatory drugs and mechanisms
The inflammatory response represents a generalized response to infection or tissue damage and is designed to remove cellular debris, to localize invading organisms and arrest the spread of infection. NSAIDS are metabol...
Tissue esterases of Lamellidens corrianus (Fresh water mussel)
Polyacrylamide gel electrophoretic (PAGE) technique was used to examine the esterase pattern of Ctenidia, Hepatopancreas, Hemolymph, Intestine, Mantle. Foot of the Lamellidens corrianus. The zymograms revealed the var...
Development of quality control parameters for the standardization of Leaves and bark of Sida acuta Burm.f
Sidha acuta Burm. f belonging to family Malvaceae is a terrestrial, erect shrub which is up to 155 cm tall. The plant is native to Mexico, Central America and Himalayan region of India but has spread throughout the tro...
Estimation of Caffeine, Niacin and Calorie Content in Tea Commonly Consumed by Dhaka City Residents
Tea is largest consuming drink in the world. Many health claims is attributed towards the tea due to its distinguished phytochemical array. Role of tea is well established as a neutraceutical and many studies elucidate...