PHYTOCHEMICAL INVESTIGATION AND CHROMATOGRAPHIC EVALUATION OF THE DIFFERENT EXTRACTS OF TUBER OF AMORPHAPHALLUS PAEONIIFOLIUS (ARACEAE)
Journal Title: International Journal of Pharmaceutical and Biological Research - Year 2010, Vol 1, Issue 5
Abstract
The present study was aimed to investigate phytochemicals present in the tuber extract of Amorphaphallus paeoniifolius and development of new solvent system for thin layer chromatography of petroleum ether and ethanol extract of Amorphaphallus paeoniifolius tuber.Initially dried powder of Amorphaphallus paeoniifolius was extracted successively in Petroleum ether, Chloroform, Methanol and water and tested for the presence of different phytoconstituents. The tuber of Amorphophallus paeoniifolius contain phytoconstituents like alkaloids, steroids, fats & fixed oil, flavonoids, tannins, proteins and carbohydrates. Among the four extracts only the Petroleum ether and methanol extract were proceed to T.L.C. For TLC, new solvent system developed for the best separation of the phytoconstituents present in the extracts. The solvent system selected for the best results of TLC were Chloroform: Benzene: Diethyl ether of the ratio 2: 2: 0.5 for petroleum ether extract and Formic acid: Acetic acid: water of the ratio 100:11:11:27 for methanol extract. TLC resulted in identification of 3 spots for each solvent system.
Authors and Affiliations
S. DE , Y. N. DEY , A. K. GHOSH*
Keywords
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