Phytochemical Screening and Antimicrobial Evaluation of Stem Bark Extract of Pseudocedrela kotschyi (Schweinf.) Herms
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 16
Abstract
Aims: This study was design to screen the stem bark extract of Pseudocedrela kotschyi for the presence of phytochemical constituents and evaluate the extract for antimicrobial activity on wide range of pathogenic bacteria and fungi species. Place and Duration of Study: Department of Pharmaceutical and Medicinal Chemistry, Usmanu Danfodiyo University Sokoto and Laboratory of Pharmaceutical Microbiology, Ahmadu Bello University Zaria, between April 2013 and Oct 2013. Methodology: Plant material was extracted with methanol and phytochemical screening carried out. Sequentially, the methanol extract was partitioned against chloroform, ethyl acetate and n-butanol to afford chloroform, ethyl acetate and n-butanol soluble fractions respectively. All fractions were evaluated against panel of pathogenic bacteria and fungi to include Methicillin Resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, Streptococcus pyogenes, Corynebacterium ulcerans, Bacillus subtilis, Escherichia coli, Salmonella typhi, Shigella dysenteriae, Klebsiella pneumoniae, Neisseria gonorrhoeae, Pseudomonas aeruginosa, Candida albicans, Candida krusei and Candida tropicalis. Results: Phytochemical screening of the extracts revealed the presence of saponins, flavonoids, tannins, glycosides, anthraquinones, steroids/terpenes as well as alkaloids. The susceptibility test of the fractions at 30mg/ml have displayed activity against S. aureus, S. pyogenes, E. coli, S. dysenteriae, P. aeruginosa, C. albicans, C. krusei and C. tropicalis at zone of inhibition ranges between 20-28mm while the MIC and MBC/MFC results showed spectrum of antimicrobial activity ranges between 2.5-10mg/ml and 5-30 mg/ml respectively. Conclusion: The activity of the extracts against S. pyogenes, E. coli, S. dysenteriae, P. aeruginosa and C. albicans, justify the traditional use of stem bark of Pseudocedrela kotschyi in the treatment of diarrhoea, dysentery and oral infection which are diseases commonly caused by these organisms.
Authors and Affiliations
Alhassan M. Alhassan, Ibrahim Malami, Musa I. Abdullahi
Keywords
Related Articles
- EP ID EP344532
- DOI 10.9734/BJPR/2014/12163
- Views 59
- Downloads 0
Changes in the Oxidative Stress Biomarkers in Liver of Streptozotocin-diabetic Rats Treated with Combretum lanceolatum Flowers Extract
Aims: The study investigated the in vivo antioxidant activity and the in vitro radical scavenging capacity of the Combretum lanceolatum Pohl (Combretaceae) flowers ethanolic extract (ClEtOH) in streptozotocin-diabetic ra...
Ameliorative Effect of Nigella sativa Oil against Paracetamol Induced Hepatic and Renal Damages in Rats
Aims: To evaluate the protective effect of Nigella sativa oil against Non-steroidal anti-inflammatory drug induced hepatic and renal damages in rats. Methodology: Study was conducted on 24 Wistar albino rats (150-200 g)...
Cytotoxicity of Enterolobium timbouva Plant Extract and Its Isolated Pure Compounds
Aims: The present study aimed to evaluate the cytotoxic activities of the aqueous alcohol extract of Enterolobium timbouva leaves as well as its isolated pure compounds. Place and Duration of Study: Department of Pharma...
Isolation and Phytochemical Characterization of Bioactive Constituents from the Seeds of Garcinia kola, Heckel (Clusiaceae)
Aim: This study was carried out with the aim of isolating and identifying the bioactive constituents of the ethyl acetate extract from G. kola seeds that was a strong inhibitor of α-glucosidase using FT-IR and GC-MS tech...
In vivo Anthelmintic Activity of Terminalia glaucescens (Combretaceae) Extracts against Gastrointestinal Nematodes of Sheep
Background: Gastrointestinal nematodes (GIN) are a major threat to sheep productivity and endanger animal welfare worldwide particularly in developing countries. They cause loss of production through mortality, weight lo...