Abstract

Piper betel called ‘paan’ has known ethnomedicinal properties and has been used as traditional herbal medicine in several countries. In the present study, an effort has been made to screen the most potent extract of Piper betel leaves which can show its ability as an antioxidant and antihaemolytic agent for alternative medicine in managing the disease. Results revealed that petroleum ether extract had high phenolic and flavonoids content followed n-butanol and aqueous extract.TLC of various extracts of Piper betel leaves depicted that the RF values of petroleum ether, n-butanol and aqueous extract was quite close to quinine standard thus representing high alkaloids content. Several concentrations of various extract ranging from 200-1000 μg/ml were tested for their reducing ability. It was observed that n-butanol fraction showed highest reducing power ability. Highest NO scavenging activity was observed in aqueous and n-butanol extracts at 1mg/ml concentration. In vitro antihaemolytic was performed by hypotonic induced method. Highest antihaemolytic activity was observed by petroleum ether and n-butanol extract. FTIR spectrum profile was generated and reported the presence of characteristic functional group.

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