Abstract

Objective: To evaluate the protective effect of ethanolic extract of Nymphaea alba Linn flowers against isoniazid induced hepatotoxicity in rats. Material and Methods: Hepatotoxicity in rats was induced by isoniazid (50mg/kg). Silymarin (50mg/kg) was used as standard drug in the study. Silymarin and two doses of ethanolic extract of Nymphaea alba Linn flowers (200 mg/kg and 400 mg/kg) were given for 31 days. Isoniazid was given in standard and test groups from 4th to 31st day of the study. All drugs were given orally. After 31 days blood samples were collected from the animals for biochemical analysis and liver tissues were subjected to histopathological examination. Results: Administration of isoniazid caused significant elevation in the levels of liver marker enzymes and oxidative stress markers in rats. Thetest extract in 200 mg/kg and 400 mg/kg doses significantly decreased isoniazid induced elevation of liver marker enzymes (AST, ALT, ALP and serum bilirubin)as well as oxidative stress markers(catalase, GSH and MDA)in rats. The changes in biochemical parameters were supported by histological profile. Conclusion: The ethanolic extract of Nymphaea alba Linn flowers (NAEE) in 200 mg/kg and 400 mg/kg dose showed protective effectagainst isoniazid induced hepatotoxicity in rats.

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