RP-HPLC Method Development and Validation for Estimation of Triamcinolone Acetonide in Injectable Suspension using USP-Type-IV Dissolution Apparatus
Journal Title: International Journal of Pharma Research and Health Sciences - Year 2016, Vol 4, Issue 6
Abstract
A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for stability indicating RP-HPLC method for estimation of Triamcinolone acetonide injectable suspension Pharmaceutical dosage form.In this method to optimize the mobile phase, various combinations of buffer, acetonitrile and water were studied on Inertsil ODS 3V, 150x4.6mm, 5 μm with a flow rate of 1.0 ml/min .The detection was carried out at 254nm.The retention time for Triamcinolone acetonide were found to 2.72 min respectively. The method was validated according to the ICH guidelines for specificity, precision, accuracy, linearity and robustness. The method showed good reproducibility and recovery with %RSD less than 2. So the proposed method was found to be simple, specific, precise, accurate and linear. Hence it can be applied for routine analysis of Triamcinolone acetonide in bulk and pharmaceutical preparations.
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