Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 11, Issue 7
Abstract
Aims: Endothelin-1 (ET-1) is a potent vasoconstrictive peptide, and its activity is mediated by the type A receptor (EDNRA). This action may play a significant role in the etiology of hypertension. There are different works that shows an association between certain polymorphisms of endothelin axis and clinical phenotype of hypertension. We describe the genetic variability +138/ex1 insertion/deletion (I/D) adenosine (A) in the ET-1 gene and polymorphism thymidine/cytosine (T/C) His323His in the EDNRA gene associated at the clinical variability in hypertensive patients. Study Design: Observational, transversal and analytical study. Place and Duration of Study: Hypertension Service at the Internal Medicine Department of Córdoba Hospital, and Biochemical and Molecular Biology Department in School of Medicine, National University of Cordoba, Argentine. Patients considered hypertensive between April 2009 and April 2010. Methodology: Were assessed 136 patients serum lipid profiles, renal and hepatic functions and were taken Thoracic X-rays, electrocardiograms, and echocardiographs. DNA extracted from circulating leukocyte were used to analyze the polymorphisms of genes by PCR-RFLP. Results: For the polymorphisms of Receptor A from Endothelin -1 studied the presence of cytosine homozygous genotype was less frequent in males (P = .02). For both genders, the same genotype was associated to low plasma alkaline phosphatase activity and cholesterol levels. The presence of thymidine nucleotide allele correlated with plasma alkaline phosphatase activity and cholesterol levels. The Thymidine allele correlated with the degree of cardiovascular compromise (r = 0.54, P= .002). For the genetic variant in the ET-1 gene, the homozygous adenine deletion was associated to normal plasma levels of glutamate/pyruvate transaminase enzyme activity, uric acid concentration, cholesterol, and Low Density Lipoprotein in hypertensive subjects without clinical risk. Conclusion: We observed a gender-specific protective effect for EDNRA gene variations, the subjects that carried the TT genotype presented more aggressive symptomatology. These results show an association between plasmatic biochemical parameters, the clinical condition, and polymorphisms in the endothelin axis genes.
Authors and Affiliations
Oscar Lassen, Jimena Herrera, Gladys Dotto, Silvia Ojeda, Alicia Garutti, Patricia Bertolotto, Sandra Tabares, Adela Sembaj
Keywords
Related Articles
- EP ID EP340237
- DOI 10.9734/BJMMR/2016/20520
- Views 35
- Downloads 0
Sleep Pattern: Preventing Factors for Alzheimer Disease
Alzheimer’s disease is investigated by scientists broadly. Although there is no definitive treatment, a better medical intervention can be assumed as a preventive medicine which postpones the onset of disease and delays...
Knowledge Attitude and Practice towards Pharmaceutical Care in Community Pharmacy in Saudi Arabia
Objective: To assess the knowledge, attitude and practice of towards pharmaceutical care for pharmacists working in community pharmacy in Alqassim region in Kingdom of Saudi Arabia. Mthedology: Two hundred twenty four ph...
Blood Loss at Caesarean Section: A Five-Year Single Centre Experience in the Niger Delta
Objective: The objective of this study is to review outcomes of our caesarean delivery in terms of: - amount of intraoperative blood loss and whether this compares favourably with blood loss at other centres. - to compa...
A Case of Drastic Improvement in a 43 Year Old Male with Severe Refractory Incapacitating Vertigo
Introduction: Vertigo is a common and distressing complaint seen in primary care. It can be due to central or peripheral causes, which should be determined right at the outset. In some cases, the vertigo is transient and...
Diabetic Ketoacidosis in Two Nigerian Adolescents with Homozygous Sickle Cell Anaemia
In the tropics where the prevalence of sickle cell anaemia (SCA) is high, reports of concurrence of sickle cell anaemia and diabetes mellitus are rare with diabetic ketoacidosis (DKA), being rarer. In this case report, w...