Plasmid Carriage and Antibiotics Susceptibility of Cultivable Bacteria Isolated from Hospital Wastewater
Journal Title: Microbiology Research Journal International - Year 2017, Vol 21, Issue 5
Abstract
Careless discharge of wastewater is another source of which the environmental health is continuously being threatened. Hospital wastewater contains clinically relevant strains. Study was undertaken on the prevalence of antibiotics resistant bacteria (ARB) in hospital wastewater and to determine the presence of plasmid and its association with antibiotic resistance expression among the bacteria using standard microbiological techniques and alkaline lysis method, respectively. Antibiotic susceptibility was determined by use of the disk diffusion method. Total heterotrophic count of 1.36 x 107 and 2.0 x 106 CFU/mL were obtained, Escherichia coli count gave 2.1 x 105 and 1.6 x 106 CFU/mL and Staphylococcal count gave 1.4 x 106 and 1.2 x 105 CFU/mL. Species of Staphylococcus, Pseudomonas, Klebsiella, Bacillus, Escherichia, Acinetobacter and Salmonella, were identified. Based on a differential staining process, the bacteria were tested against some commonly used antibiotics. A high profile of multi-antibiotics resistance was displayed among the bacteria. The percentage of resistance to the antibiotics were COT - 15%, AUG - 15%, GEN - 11%, TET - 10%, OFL - 3%, AMX - 10%, NIT - 5%, NAL - 6%, CXC - 10%, CHL - 6%, ERY - 3% and STR - 6%. Approximately 58% of the bacteria carried plasmids. Plasmid band size of 23.1 kbp was common among the bacteria. Resistance was observed in bacteria with plasmids as well as in those without plasmids. On curing, the organisms showed reduced resistance to the antibiotics while Pseudomonas aeruginosa and Bacillus sp. maintained their resistant pattern. Hospital wastewater contains loads of antibiotic resistant bacteria whose resistance in part are carried in plasmid. It contributes to environmental contamination with clinically important strains of microorganisms. The practice of releasing hospital wastewater into the environments should be strongly monitored and prohibited as the environment has a lot to do in our functioning and metabolic efficiency and therefore should be kept clean.
Authors and Affiliations
M. P. Uko, S. C. U. Nwachukwu, S. I. Umana
Keywords
Related Articles
- EP ID EP316045
- DOI 10.9734/MRJI/2017/36417
- Views 81
- Downloads 0
Prevalence and Microbial Resistance of Uropathogenic Bacteria Isolated from Neonatal and Pediatric Patients in Western of Iran
Backgrounds: Urinary tract infections (UTIs) are the most common bacterial infections disease among children, pediatric and neonatal patients. These infections are found frequently in children. The global evolution of an...
Ability of Trichoderma harzianum from Semi Arid Soils to Enhance Antioxidant Defense of Maize Seedlings under Water Stress
Aim: Determine the effect of different inoculum concentrations of T. harzianum from semi - arid soils on the activity of antioxidant enzymes of maize seedlings under water stress. Methods and Results: This study employe...
Biochemical and Molecular Characterization of Bacterial Wilt Disease of Banana and Evaluation of their Antibiotic Sensitivity
Aims: Isolation and characterization of bacteria from the bacterial wilt disease of banana and evaluation of their sensitivity. Study Design: This study was an experimental laboratory design. Place and Duration of Study...
Assessment of Bacteriological Quality of Tono and Vea Dams Water in the Upper East Region, Ghana
Aim of the Study: This study was undertaken to assess the bacteriological quality of water from the Tono and Vea dams. Study Design: Water samples from both Tono and Vea irrigation dams in the Upper East Region of Ghana...
Viability of Fungal Spores Isolated from Sorghum Grains Sampled from the Field, Market and Different Storage Facilities in the Six Agro-ecological Zones of Nigeria
Nutritional quality, organoleptic attributes and food safety are usually compromised by the presence of Microscopic fungi or fungal spores in food and feeds that humans and animals solely rely on. It is therefore intende...