Plasmid expression vector encoding sFLT-1 receptor (psFLT-1) inhibits angiogenesis and L1 tumour growth
Journal Title: Współczesna Onkologia - Year 2006, Vol 10, Issue 4
Abstract
Antiangiogenic gene therapy is based on genes encoding protein factors that suppress a process of new vessels formation – angiogenesis. It is common knowledge that angiogenesis is a crucial point of the tumour growth and metastasis formation. Therefore, many new anticancer strategies and drugs are focused on the inhibition of angiogenesis. psFLT-1 is a nonviral, plasmid expression vector encoding soluble form of vascular endothelial growth factor receptor VEGFR1 (sFLT-1). sFLT-1 gene encodes only extracellular domain of the VEGFR1 and specifically blocks proangiogenic activity of VEGF. The studies were performed on laboratory animals subcutaneously or intraperitoneally injected with mouse L1 fibrosarcoma cells or their psFLT-1 transfectants. The new vessels formation, and tumour growth, and survival time were evaluated on tested animals. The obtained outcomes revealed that psFLT-1 expression vector efficiently inhibits pVEGF induced angiogenesis as was measured by two in vivo angiogenesis tests. It was also shown the inhibitory psFLT-1 effect on L1 tumour growth and survival time of the L1 intraperitoneally injected animals. The prolonged survival time of L1/psFLT-1 animals was clearly observed. Finally, the clinical application of psFLT-1 gene construct was taken under consideration. At present, psFLT-1 based antiangiogenic clinical trial is in progress in the Centre of Oncology in Warsaw.
Authors and Affiliations
Maciej Małecki, Katarzyna Gromek, Małgorzata Przybyszewska, Przemysław Janik
Keywords
Related Articles
- EP ID EP91148
- DOI -
- Views 63
- Downloads 0
Postępy w leczeniu wczesnego raka piersi – najważniejsze doniesienia z 2005 r. – sprawozdanie z konferencji
Adjuvant endocrine therapy in breast cancer
Endocrine therapy is the most important component of adjuvant therapy for patients with hormone receptor-positive breast cancer, regardless of age and menopausal status. Tamoxifen improves survival in premenopausal and...
Activity of β-galactosidase and α-mannosidase in the serum of patients with colon adenocarcinoma
Exoglycosidases β-galactosidase (GAL) and α-mannosidase (MAN) take part in the synthesis of glycoconjugates in the endoplasmatic reticulum and Golgi apparatus, as well as their degradation in the lysosomes. An increase...
Fruits and vegetables consumption and the risk of breast cancer
Background: Breast cancer remains a worldwide public health concern for women of all ages. The disease represents the second leading cause of cancer death among women. Results of most epidemiological studies have d...
Ból w przebiegu choroby nowotworowej u dzieci i młodzieży
W Polsce rocznie rozpoznaje się 1100–1300 nowych zachorowań na nowotwory w populacji wieku rozwojowego. Najczęściej u dzieci występują białaczki, guzy mózgu i chłoniaki, stanowiąc 59,3% wszystkich nowotworów dziecięcyc...