Plasmodium vivax Detected on Anophles punctulatus Using ELISA: A Vector Confirmation Study in Tamraw District, A Remote Area With High Incidence of Malaria, West Papua Province-Indonesia
Journal Title: Journal of Medical Science And clinical Research - Year 2015, Vol 3, Issue 7
Abstract
Background: Tamraw District is a new administrative district of West Papua Province with high incidence rate of malaria. The aim of the study was to detect Plasmodiumsp in Anophelessp using ELISA assay in Tamraw District. Material and Methods: Mosquitoes sample collection was caried out using human landing collection method in Anopheles sp active period (18.00-06.00). The identification of species was done in situ, immediately after collection. The sandwich ELISA for detection P.falcifarum and P.vivax sporozoit was carried out. Result: The total Anopheles punctulatus groups collected in this study were 179, consist of An. farauti (15%), An.koliensis (7%) and An.punctulatus (78%). The active period peak of Anopheles punctulatus group was on 19.00-20.00 in indoor area. The ELISA result shows that Plasmodium vivax was detected on of Anopheles punctulatus samples with the sporozoit index 3,27%. Conclusion: Base on sporozoit detection using ELISA, Anopheles punctulatus in Tamraw District West Papua confirmed as a malaria vector
Authors and Affiliations
Hana Krismawati
Keywords
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