PLUM POX VIRUS ELIMINATION FROM SOME PLUM CULTIVARS BY IN VITRO CULTURE
Journal Title: FRUIT GROWING RESEARCH - Year 2016, Vol 0, Issue
Abstract
For a good management of viral diseases along with the development of sensitive diagnostic techniques substantial attention should be paid to the biotechnological methods for viruses elimination from plants. The experiments conducted at RIFG Pitesti-Romania tried to establish the optimal parameters that would lead to increase efficiency of in vitro culture for Plum pox virus elimination from ‘Carpatin’ and ‘Pescăruș’, plum cultivars. The meristems divided into two groups by size: 0.2-0.4 mm-type A and 0.8-0.9 mm-type B were regenerated on MS and LF basic media with 0.01 to 0.1 mg/l IBA and 0.2 mg/l GA3 hormonal balance. Multiplication of shoots was developed on the same basal culture media with adapting hormonal balances according to stage. The obtained results showed that the explants size has influenced both the regenerative capacity and degree of virus elimination, irrespective of cultivars and basic media used. Thus in case of ‘Carpatin’ cultivar with type B explants, regeneration rate increased to 95%, but the virus free plants obtained was only 53.57% after six subcultures. With the same cultivar in case when we used type A explants the regeneration capacity obtained was only 33%, but increased the percent of virus free plants up to 82.14 %. The leaf samples of the obtained plantlets of each cultivar were tested for PPV by DAS –ELISA serological technique.
Authors and Affiliations
Catiţa Plopa, Valentina Isac, Mădălina Butac
Keywords
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