Poly (acrylamide) hydrogels with improved thermal, morphological properties and swelling behaviour: Influence of lipase immobilization onto hydrogel
Journal Title: International Journal of Engineering and Science Invention - Year 2017, Vol 6, Issue 10
Abstract
Presenting study have been focused on redesigning poly(acrylamide) superabsorbents with high swelling performance via organic structure additions, interpenetrating network formation and hydrophilic reactive hydrogel formation as well as enzyme addition. Poly(acrylamide) hydrogels (PAAm) prepared by immobilization of the lipase enzyme in the form of reversibly bound material, were studied in terms of swelling, degree of loading and releasing enzyme, enzyme activity properties, thermal stability and surface morphology. The swelling behaviors of lipase-immobilized and lipase-removed hydrogels (LIRHs) were investigated as a function of time. Swelling data was fitted to various diffusion models and model parameters were evaluated using a regression technique. Model analysis indicated that the swelling transport followed Fickian and nonFickian mechanisms as a function of time. The activity recovery of immobilized lipase into the PAAm hydrogel network reached 78% for the 3.0% lipase enzyme and also, more than 7.0% lipase loading into the network structure caused a decrease not only to the bound enzyme activity but also to the immobilization yield of the enzyme.
Authors and Affiliations
Didem Saloglu, Nazli Ozcan, Gokhan Temel
Keywords
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