Poly-𝛽-hydroxybutyrate degradation by Aspergillus fumigates isolated from soil samples collected from Jeddah, Saudi arabia
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 1
Abstract
Poly-β-hydroxybutyrate acid (PHB) is thermoplastic biopolymers, synthesized by some bacterial genera under stress conditions. The degradation of PHB was detected in Petri dish by formation of a clear zone around the fungal colonies due to production of depolymerase enzyme which has interesting role in PHB degradation process. The most active PHB degrader fungi was selected and identified as Aspergillus fumigates using morphological characters. The highest PHB degradation in Petri dish by A. fumigates was at pH 5, 30ᵒC and 7days. In liquid medium, degradation by A. fumigates was studied using enzyme assay method (U/ml). All the experiments were performed enzyme activities were monitored. After 3 days of incubation, maximum PHB depolymerase production was at pH 5 and 30°C. In conclusion, PHB can be degraded in solid and liquid medium using fungal depolymerase enzyme.
Authors and Affiliations
Magda Mohamed Aly1, Tahani Ali Mohamed Jaar, Fardus M. Bokhari
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