Polymorphism of CD36 gene, carbohydrate metabolism and plasma CD36 concentration in obese children. A preliminary study
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2012, Vol 66, Issue 0
Abstract
Introduction: CD36 may play an important role in removal of oxidized LDLs from plasma, protein glycation, the pathogenesis of insulin resistance, type 2 diabetes, and diabetic micro- and macroangiopathy. Some reports have pointed to decreased expression of macrophages in association with mutations of the CD36 gene in hyperglycemic and obese subjects. The aim of the study was to search for an association between CD36 gene polymorphism and carbohydrate metabolism disturbances or variability of plasma soluble CD36 concentrations in obese children. Material/Methods: The study included 60 children aged 10 to 15 years: 30 with (study group) and 30 without (control group) obesity. Each patient’s glycated hemoglobin, weight, height, waist and hip circumference, and systolic and diastolic blood pressure were measured, BMI, WHR and MAP were calculated, and oral glucose tolerance test was performed with glucose and insulin concentration measurements. Amplicons of exons 4–6 of CD36 were studied using DHPLC technique. The PCR products with alterations were bidirectionally sequenced. Plasma concentrations of human antigen CD36 was measured using a commercially available enzyme-linked immunosorbent assay (ELISA). Results: We found two intronic alterations: IVS3-6 T/C (rs3173798) and IVS4-10 G/A (rs3211892), one nonsynonymous substitution: G367A (Glu123Lys, rs183461468) in exon 5 and two synonymous transitions in exon 6: G573A (Pro191Pro, rs5956) and A591T (Thr197Thr, rs141680676). There were no significant differences in any biochemical or morphometric parameters between genotype groups. Discussion: The polymorphisms of the studied fragment of CD36 are not associated with carbohydrate metabolism disturbances or the variability of plasma soluble CD36 concentrations in obese children, but further research is necessary to assess their functional implications.
Authors and Affiliations
Monika Rać, Beata Krupa, Barbara Garanty-Bogacka, Małgorzata Syrenicz, Krzysztof Safranow, Violetta Dziedziejko, Grzegorz Kurzawski, Maria Olszewska, Michał Rać, Dariusz Chlubek
Keywords
Related Articles
- EP ID EP66832
- DOI -
- Views 154
- Downloads 0
Mechanisms of programmed cell death of effector T lymphocytes
T cell adequate function is critical for defense against pathogens. Transient disruption of T cell homeostasis occurs when primarily naive cells undergo antigen-driven expansion and acquire effector functions. Effector T...
Activity of N-acetyl-β-D-hexosaminidase in the saliva of children with type 1 diabetes
Background/Aim: Type 1 diabetes is one of the most common chronic diseases in children. The aim of the study was to evaluate the catabolism of glycoconjugates in saliva of children with type 1 diabetes, by measurement of...
Białko C-reaktywne (CRP) jako czynnik diagnostyczny i prognostyczny w nieswoistych chorobach zapalnych jelit
Genetyczne i immunologiczne uwarunkowania odpowiedzi na dopęcherzową terapię BCG w raku urotelialnym pęcherza moczowego nienaciekającym błony mięśniowej
Rak pęcherza jest jednym z najczęściej występujących nowotworów złośliwych. W Polsce w 2010 r. zachorowało 6296, a zmarło 3110 osób. Immunoterapia BCG (Bacillus Calmette-Guérin) jest obecnie najskuteczniejszą terapią adi...
Molekularne podstawy interakcji międzylekowych w terapii nowotworów jelita grubego
Szybki postęp w dziedzinie chemioterapii nowotworów zaowocował wprowadzeniem do codziennej praktyki klinicznej wielu cennych leków o działaniu przeciwnowotworowym, umożliwiając skuteczniejsze leczenie chorych, również po...