Polymorphisms of the prion protein Gene in Iranian Holstein and Najdi and Sarabi cattle breeds
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2013, Vol 6, Issue 11
Abstract
Polymorphisms of the 23-bp in/del in the promoter region and the 12-bp in/del in intron 1 of the prion protein gene, which are shown to be associated with resistance to bovine spongiform encephalopathy (BSE), were assessed in the Nova Soscotia Agricultural College at Canada from March to September 2010. We studied polymorphyism of the two loci among samples of two Iranian cattle breeds including Najdi, Sarabi and Iranian Holstein. Whole blood samples were collected randomly. Selected cows DNA were extracted by Diatom kit. Holstein breed had significantly different allele, genotype and haplotype frequency distributions compared with the two native breeds for both polymorphic sites, except for the 23-bp in/del which was comparable with the Sarabi breed. The frequencies of the protective 23-bp insertion allele (23+) were 0.28, 0.15 and 0.33 in the Sarabi, Najdi and Holstein breeds, respectively. The corresponding values for the low susceptibility 12-bp insertion allele (12+) were 0.92, 0.89 and 0.48, respectively. The frequencies of this allele in the two native breeds are at the upper limit of most breeds studied so far. The allele and haplotype frequency distributions of the Najdi breed resembled that of the published data on other B. indicus cattle. It is not possible to predict the degree of susceptibility of the native breeds to BSE based on the frequencies of these two polymorphic sites because of uncertainties regarding the relative contribution of each site to the overall resistance to BSE and the presence of breed-specific background genes affecting this trait.
Authors and Affiliations
H. Roshanfekr| Associate Professor of Genetics and Animal Breeding. Dept. Animal Science. Faculty of Animal Science and Food Industrial. Ramin Agricultural University and Natural Resources, Mollasani, Ahwas. Iran. roshanfekr_hd@yahoo.com, A. Farid| Professor of Genetics and Animal Breeding. Department of Plant & Animal Science. Nova Scotia Agricultural College. Truro, Nova Scotia, Canada. email: Hfarid@nsac.cam
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