Possible Mechanism of Antispasmodic Action of Garcinia kola Seed Extract on Isolated Guinea Pig Ileum
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2015, Vol 6, Issue 7
Abstract
Aims: Garcinia kola is used in West African countries for the treatment of various ailments such as cough, tooth decay, asthma and menstrual cramps. The inhibitory effect of Garcinia kola seed extract (GKE) on drug-induced contractions was studied on iliac smooth muscle preparations of guinea pig to ascertain the validity of the use of Garcinia kola in traditional medicine and to elucidate its possible mechanism of action. Place and Duration of Study: The study was done in Post Graduate Laboratory, Department of Pharmacology, College of Medical Sciences, University of Calabar, Calabar-Nigeria, between November 2013 and April 2014. Methodology: The antispasmodic influence of GKE (0.02 – 1 mg/ml) on acetylcholine, histamine and potassium chloride -induced contractions were carried out. The effect of GKE in a Ca2+-free Tyrode medium and in the presence of adrenergic antagonists was also investigated. Results: The results revealed that GKE inhibited or attenuated the spasmogenic effects of histamine and potassium chloride in a dose-dependent manner and shifted their log. dose-response curves to the right, with pA2 values of 2.09±0.06 and 3.25±0.07 respectively. Pre-administration of propranolol, prazosin or labetalol had no attenuating influence on the antispasmodic effect of GKE. Iliac smooth muscle responses to cumulative increased [Ca2+] in a depolarizing bathing medium and in a Ca2+- free Tyrode solution were also blocked. Comparative antispasmodic potencies indicated that papaverine and aminophylline were more potent than the extract. Conclusion: These findings suggest that Garcinia kola seed extract acts neither via cholinergic nor adrenergic receptor mediation, but may involve interference with Ca2+ mobilization, thus sharing with papaverine and/or aminophylline similar mechanism(s) of action.
Authors and Affiliations
P. M. Udia, D. U. Owu, O. J. Ogbonna, L. P. Takem
Keywords
Related Articles
- EP ID EP347205
- DOI 10.9734/BJMMR/2015/14210
- Views 53
- Downloads 0
A Rare Case of Acute Psychosis as an Isolated Manifestation of Extrapontine Myelinolysis
Aims and Background: Central pontine myelinolysis (CPM) and extrapontine myelinolysis (EPM) are recognized as osmotic demyelination syndrome (ODS). ODS is pathologically characterized by non-inflammatory demyelination of...
A Case Report of Invasive Meningococcal Disease in an Adult without Identifiable Risk Factors
Meningitis is the most common manifestation of invasive meningococcal disease (IMD), occurring in approximately 50% of all cases. It is characterized by sudden onset, very rapid progression, and a high case fatality rate...
Transcutaneous Electrical Nerve Stimulation (TENS) Reduces Pain and Drug Intake in the Immediate Postoperative Period Following Proximal Femoral Fractures
Aim: The aim of this study was to evaluate the action of TENS on the pain threshold and on the intake of analgesic drugs in patients with proximal femoral fractures. Study Design: Randomized, double-blind, placebo-contro...
Vitamin D Status and Contributing Factors in Patients Attending Three Polyclinics in Benghazi Libya
Background: About one billion people in the world suffer from vitamin D deficiency or insufficiency. The consequences of low vitamin D level include increased risk of some cancers, cardiovascular diseases, and type one d...
Combined Therapy of Moringa oleifera and Ocimum gratissimum Reversed Testicular Damage in Diabetic Rats
Introduction: Testis is an important male reproductive and endocrine organ whose structure and function are altered in diabetes complicated disorders. Aim: This study evaluated the protective effect of Moringa oleifera (...