Possible role of sialylation of retinal protein glycans in the regulation of electroretinogram response in mice
Journal Title: International Journal of Ophthalmology - Year 2017, Vol 10, Issue 8
Abstract
"AIM: To evaluate if the nature, degree and extent of Siaα2-3-/Siaα2-6-sialylation of retinal protein glycans plays a possible role in the development and regulation of electroretinogram response (ERG) in mice. METHODS: Proteins extracted, from retinae of postnatal day 2 (PN2), PN7, and PN14 wild type (wt) and retinal degeneration 1 (rd1) mice were quantified, labeled and used for lectin-microarray profiling with immobilized lectins which recognize a wide range of N-/O-glycans. Net fluorescence intensities of lectin-ligand complexes were measured and images of fluorescent lectin-microarrays were acquired. From the binding curves between each lectin and protein extracts from PN14 wt and PN14 rd1 mice retinae, the protein concentration was selected to determine optimum signal intensity for lectin-ligand binding. Mean±SEM values of proteins and fluorescence-intensities of lectin-ligand-complexes between 45 lectins and 36 protein extracts from wt and rd1 mice retinae were compared for significance of differences. RESULTS: Comparison of the progressive relative changes in the sialylated glycans of retinal proteins from wt and rd1 mice showed that Siaα2-3Galβ1-4GlcNAc-glycans (but not Siaα2-6-glycans) were detectable and quantifiable from the retinal-proteins of PN7 and PN14 wt and rd1 mice. Siaα2-3-sialylation of retinal-protein Gal/α-linked-Gal-glycans was significantly increased with age in PN7 and PN14 wt and less so in PN14 rd1 mice. Siaα2-3-/Siaα2-6-sialylation of retinal-protein Gal/α-linked-Gal-glycans was absent in PN2 wt and rd1 mice. Comparison of published ERG responses of wt and rd1 mice retinae with degree of Siaα2-3-sialylation of retinal-protein-glycans showed that PN2 wt and rd1 mice lack both the ERG response and Siaα2-3-/Siaα2-6-sialylation of retinal-protein Gal/α-linked-Gal-glycans; rd1 mice with relatively lower Siaα2-3-sialylation of retinal-protein Gal/α-linked-Gal-glycans showed aberrant ERG response; and wt mice with significantly higher Siaα2-3-sialylation of retinal-protein Gal/α-linked-Gal-glycans showed normal ERG response. CONCLUSION: Degree of Siaα2-3-sialylation of glycans possibly regulates ERG function in mice."
Authors and Affiliations
Satpal Ahuja
Keywords
Related Articles
- EP ID EP595401
- DOI 10.18240/ijo.2017.08.05
- Views 57
- Downloads 0
The reason for the amelioration of N-methyl-N-nitrosourea- induced retinitis pigmentosa in rats by hydrogen-rich saline
"AIM: To investigate the effects of hydrogen-rich saline (HRS) on microglia activation and Sirtuin type 1 (Sirt1) in rats with N-methyl-N-nitrosourea (MNU)-induced retinitis pigmentosa (RP). METHODS: Rats were divided in...
Comparison of OA-2000 and IOL Master 500 using in cataract patients with high myopia
This study was designed to compare optical biometry measurements and predicted refraction in cataract patients with high myopia [axial length (AL) ≥26 mm] using OA-2000 and IOL Master 500. Ocular biometry measurements we...
Ophthalmic community perception of new medication needs
"AIM: To survey ophthalmologists (who have participated previously in clinical research) and ophthalmic industry professionals (who have been involved in ocular research and development) to indicate perceived needs for n...
Differentially expressed miRNAs in premature infants with retinopathy-a bioinformatics analysis
"AIM: To reveal the role of miRNAs in retinopathy of prematurity (ROP) by bioinformatics analysis. METHODS: The raw data of this study came from the researches of Wang et al and Zhao et al who analyzed the microRNA (miRN...
Focal choroidal excavation complicated with choroidal neovascularization in young and middle aged patients
"AIM: To investigate the clinical and optical coherence tomography (OCT) features of focal choroidal excavation (FCE) complicated with choroidal neovascularization (CNV) in young and middle aged patients. METHODS: We per...