POTENTIAL INFLUENCE OF CULTURE MEDIUM CONSISTENCE AND SILVER NITRATE ON MICROPROPAGATION OF SOLANUM VIARUM, AN ANTICANCER MEDICINAL PLANT
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2015, Vol 4, Issue 4
Abstract
An understanding of optimal nutrient concentration could lead to increased growth and also could evoke maximum morphogenesis in vitro more effectively. Experiments were conducted to determine suitable combination of AgNO3 concentration and artificial nutrient media to stimulate in vitro growth of Solanum viarum. All the four types of basal media such as Murashige and Skoog (MS), Schenk and Hilderbrandt (SH), Gamberg media (B5) and K S medium were supplemented with 0 to 0.6 mg/L AgNO3, 2mg/L BAP, 0.5mg/L IAA, 3% sucrose and 0.8% of agar. Leaf explants supplemented with M S media responded the best at 0.4 mg/L AgNO3 with maximum mean shoot lengths (37.3 ± 0.46) and also with very high regeneration frequency (95%). Though maximum shoot lengths (10.2 ± 0.27 cm) were obtained in KS medium at 0.4mg/L AgNO3, the plantlets were found to be fragile and chloretic and were found to be very less feasible in the subsequent cultures. Explant had shown minimum response in SH medium and also nil response was recorded in case of B5 medium. So, MS media at 0.4 mg/L AgNO3 concentration was found to be more consistent media for conserving and propagating Solanum viarum under in vitro conditions.
Authors and Affiliations
Sujana Papani*| Department of Botany & Biotechnology, P.V.K.N Government College, Chittoor, Andhra Pradesh., Naidu C. V| Department of Biotechnology, Dravidian university, Kuppam, Andhra Pradesh.
Keywords
Related Articles
- EP ID EP3588
- DOI -
- Views 347
- Downloads 24
ANALYSING THE RATIONALITY OF ANTIBIOTICS IN COMPARING THE DIFFERENT DEPARTMENTS IN RMMCH
They are many problems which are caused by healthcare system. This study was conducted to know and determine the rational use of antibiotics;drugs were analyzed for appropriateness in dosage, duration of therapy and dr...
COMPARITIVE EVALUATION OF TISSUE GLYCOGEN AND SALIVARY PH IN CHRONIC GINGIVITIS AND CHRONIC PERIODONTITIS PATIENTS
BACKGROUND: The inflammation of gingiva is known as gingivitis with microorganisms present in the gingival sulcus, being the major cause for this pathologic condition. Periodontitis is a chronic inflammatory disease whic...
EFFECT OF CIGARETTE SMOKING ON HAEMATOLOGICAL PARAMETERS: INFLUENCE OF NQO1 GENE POLYMORPHISM
Cigarette smoking carries higher risks for most of the chronic diseases. It also has chronic and acuteeffects on the haematologic system.NQO1 catalyzes highly toxic quinones derived from tobacco smoking to less toxic hyd...
NEUROPATHY TARGET ESTERASE; ITS ROLE IN PHOSPHATIDYLCHOLINE REGULATION AND IMPLICATIONS FOR PATHO-PHYSIOLOGY
Eukaryotic cells control the levels of their major membrane phospholipid, phosphatidylcholine (PC), by balancing synthesis with degradation via deacylation to glycerophosphocholine(GPC). Neuropathy target esterase (NTE)...
TIBIAL PLATEAU FRACTURES–OUTCOME COMPARISON BETWEEN AUTOGRAFT AND TRICALCIUM PHOSPHATE