POTENTIAL OF RESVERATROL PRODUCTION WITH BIOTRANSFORMATION BY YEAST USAGE
Journal Title: International Journal of Bio-Technology and Research (IJBTR) - Year 2017, Vol 7, Issue 1
Abstract
Resveratrol, a phytoalexin, excreted naturally by several plants such as grape, Japanese knotweed and peanut. Also, many of them contain some kinds of glycosides such as piceid which shows less bioactivity. β-Glucosidase is an enzyme that hydrolyzes β-glycosidic bonds between the reducing side of glucose and an aryl, alkyl aglycone or oligosaccharide. Therefore, β-glucosidase could be used for deglycosylation of piceid into resveratrol for a commercial application. In this research, hydrolysis of piceid in Polygonumcuspidatum to resveratrol by a patented microorganism Dekkerabruxellensis (BCRC 920084), secreting β-glucosidase was investigated. In addition, β-glucosidase for enhancing biotransformation efficiency was multiple used within whole cells under condition of liquid suspension. Then, a series of enlarging experiments were followed by 50-mL, 3-L and 10-L operation volume and the conversion rate was average 92.55 ± 9.66% after 5 times reused efficiency.
Authors and Affiliations
HSIAO-PING KUO, YI-SHENG LIN, JINN-TSYY LAI, SHYUE-TSONG HUANG
Keywords
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