Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats
Journal Title: Journal of Applied Veterinary Sciences - Year 2017, Vol 2, Issue 1
Abstract
To examine the effect of osteopontin inhibition by tranilast on liver fibrosis, four groups of rats were used throughout this study. Group I (Control group): rats received the solvent. Liver fibrosis was induced in Groups II, III, and IV by thioacetamide (TAA; 200mg/kg, ip) twice weekly for 6 weeks. Group II served as (TAA group). Groups III and IV (Treatment groups): rats were given tranilast for 6 weeks after TAA discontinuation. Liver osteopontin (OPN), transforming growth factor-β (TGF-β1), tumour necrosis factor-alpha (TNF-α), alpha-smooth muscle actin (ɑ-SMA)), reduced glutathione (GSH), superoxide dismutase (SOD) and lipid peroxidation (MDA) were measured. Additionally, the expression of α-smooth muscle actin (SMA) and caspase (Cas)-3 were assigned immunohistochemically. Treatment with tranilast prevented the development of hepatic fibrosis and the activation of stellate cells, and down-regulated the expression of genes for osteopontin and osteopontin-target molecules, including TGF-β and TNF-α and α-SMA. Tranilast significantly decreased MDA and increased levels of GSH, and SOD. Our findings suggest that targeting osteopontin with tranilast represents a new model of therapy for liver fibrosis.
Authors and Affiliations
Ramadan A. , Nehal A. Afifi , Nemat Z. Yassin, Rehab F. Abdel-Rahman , Sahar S. Abd El-Rahman, Hany M. Fayed
Keywords
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- EP ID EP677177
- DOI 10.21608/JAVS.2017.62129
- Views 333
- Downloads 0
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