Potential use of Quercus infectoria gall extracts against urinary tract pathogenic bacteria
Journal Title: International Journal of Research in Pharmacology & Pharmacotherapeutics (IJRPP) - Year 2014, Vol 3, Issue 3
Abstract
Galls of Quercus infectoria have been traditionally used by Malaysian women as a remedy after childbirth and also to prevent various infections including urinary tract infection (UTI). This study aimed to evaluate the in vitro antibacterial activity of Q. infectoria gall extracts against several bacterial pathogens of the urinary tract. Methanol and aqueous extracts of Q. infectoria galls were screened against 4 Gram-positive bacteria (Staphylococcus saprophyticus ATCC 49907, Streptococcus agalactiae ATCC 13813, Streptococcus pneumoniae ATCC 27336, and Enterococcus faecalis ATCC 29212) and 4 Gram-negative bacteria (Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 1706, Proteus mirabilis ATCC 12453 and Proteus vulgaris ATCC 49132) using disc diffusion method. The minimum inhibitory concentrations (MIC) were determined using the two-fold serial micro dilution technique at concentrations ranging from 0.01 mg/ml to 10 mg/ml. Minimum bactericidal concentration (MBC) were determined by sub culturing the wells which showed no turbidity after overnight incubation at 37°C on the agar plate. Both extracts displayed similar inhibitory effects against each tested bacteria at a concentration of 5 mg/disc except for P. vulgaris, E. coli and K. pneumoniae. The extracts were also considered bactericidal against the tested bacteria (undetermined for S. pneumoniae) based on calculated MBC/MIC ratio. Gas Chromatography-Mass Spectrometry (GC-MS) analysis showed that the major compound in both extracts was pyrogallol. These data suggested that Q. infectoria galls are potentially effective as an antibacterial agent for the prevention and treatment of UTI.
Authors and Affiliations
Nur Saeida B
Keywords
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