Power of different microsatellite panels for paternity analysis in sheep
Journal Title: Animal Science Papers and Reports - Year 2016, Vol 34, Issue 2
Abstract
The aim of this work was to develop and to test PCR-based suitable microsatellite marker panels for paternity testing in sheep. Blood samples were collected from 212 Kivircik sheep (24 rams and their 188 offspring) raised in five farms were included in the controlled mating programme and genotyped with 20 microsatellite markers. Different combinations with multiplex microsatellite groups were established, providing seven microsatellite panels to determine the power of paternity testing. A total of 318 alleles were detected across the 20 loci investigated. The average PIC value (0.80) obtained from all loci indicated that these microsatellite panels were very polymorphic and highly informative. The highest and lowest average number of alleles were observed in Panel 3 and Panel 1, respectively, among the formed panels. Probability of identity (PI) values varied from 0.102 to 0.020. The power of discrimination (PD) values ranged from 0.678 and 0.979. Combined probability of identity (CPI) value ranged between 1.20x10-26 (Panel-7) and 7.24x10-6 (Panel-1). The lowest and highest combined probability of exclusion values were recorded for Panel-1 (0.931912000) and Panel-7 (0.999998870), respectively, as it could have been expected. According to the study results panels 4 and 5, formed with fewer microsatellite markers for paternity tests, were cheaper and more practical than the other panels in Kivircik sheep.
Authors and Affiliations
Onur Yilmaz
Keywords
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