A novel pig gene, FASTK, differentially expressed in the muscle tissues from Wujin and Large White pigs
Journal Title: Animal Science Papers and Reports - Year 2010, Vol 28, Issue 4
Abstract
The mRNA differential display technique was used to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the rapid amplification of cDNA ends (RACE) method. The open reading frame of this gene encodes a protein of 542 amino acids, which is homologous with the Fas-activated serine/threonine kinase (FASTK) of seven species: cattle 92%), sumatran orangutan (91%), chimpanzee (90%), rhesus (85%), dog (92%), mouse (87%) and human (91%). This newly identified gene was respectively defined as the swine FASTK gene and has been assigned GeneID: 100233181. The phylogenetic tree analysis revealed that the porcine FASTK has a closer genetic relationship with the FASTK of cattle. The tissue expression analysis indicated that the FASTK gene is differentially expressed in various porcine tissues. The presented investigation is the first to establish the primary foundation for further research on the swine FASTK gene.
Authors and Affiliations
Yan Dawei, Bi Baoliang, Liu Yonggang
Keywords
Related Articles
- EP ID EP71503
- DOI -
- Views 87
- Downloads 0
Concentration of harmful gases in poultry and pig houses
A common chicken broilers rearing house and fattening pig barn were monitored for harmful gases concentration (NH3, CO2, NO2 and CH4). In chickens the concentrations of NH3 rose during fattening periods in all seasons of...
Detection of difficult conceptions in dairy cows using selected data mining methods
In the present study, the detection of difficult conceptions in dairy cows using selected data mining methods – na ve Bayes classifier (NBC) and regression and classifications trees (CART) is presented. The set of 11 dia...
Effect of mutation in MC4R gene on carcass quality in Pulawska pig included in conservation breeding programme
In addition to highly productive breeds of pigs, Polish breeders keep local pigs subject to the conservation programme including Pulawska (P) pig. Analysis of records spanning 20 years showed that lean...
Glucose infusion response to some biochemical parameters in dairy cows during the transition period
The aim of this study was to evaluate the effect of pre-partum versus post-partum periods on the biochemical profile in dairy cows. The effect of glucose tolerance test (GTT) on glucose (Gluon-farm) and β-Hydroxybutyrate...
Body condition score loss, hepatic lipidosis and selected blood metabolites in Holstein cows during transition period
The aim of this study was to investigate the relationship between the loss in body condition score (BCS) and loss (ΔBCS), energy balance (EB), hepatic lipidosis and blood serum concentration of non esterified fatty acids...