Prediction of B cell epitopes of CD36 and preparation of MAP
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 8
Abstract
Objective To analyze the structure of CD36, search the possible B cell epitopes and prepare multi-antigen peptides(MAP) with B cell epitopes, so as to provide a preliminary experimental basis for the preparation of CD36 antibodies using MAP with B cell epitopes. Methods The potential B cell epitopes of CD36 were analyzed by bioinformatics methods, including physical and chemical properties, secondary structure, potential phosphorylation and glycosylation sites. Eight-branch MAP with CD36 B cell epitopes were synthesized by FMOC using polylysine as the core matrix. The purity of MAPs was analyzed by reverse high-performance liquid chromatography chromatography(RP-HPLC), and the molecular weight of MAPs was determined by mass spectrometry. Results CD36 is a stable and hydrophilic alkaline protein, with multiple phosphorylation and glycosylation sites and strong antigenicity, and its secondary structure is mainly characterized by irregular curls. Four potential B cell epitopes were obtained and 4 MAPs containing potential B cell epitopes were prepared. RP-HPLC analysis showed that the purity of the MAPs were above 85%, and the molecular weight of 3 MAPs was consistent with the expected theoretical molecular weight. Conclusion CD36 on platelet has strong antigenicity. MAPs containing CD36 B cell epitopes can provide the experimental basis for the preparation and related research of CD36 antibodies.
Authors and Affiliations
Jing LIU, Xiuzhang XU, Haoqiang DING, Jing DENG, Jiali WANG, Yangkai CHEN, Wenjie XIA, Xin YE
Keywords
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- EP ID EP744444
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.08.002
- Views 46
- Downloads 0
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