Whole-genome polymorphism of CD36 by third-generation sequencing technology
Journal Title: Chinese Journal of Blood Transfusion - Year 2025, Vol 38, Issue 5
Abstract
[Objective] To analyze CD36 gene by PacBio Sequel Ⅱ the third-generation sequencing technology (TGS), including non-coding sequence, and to investigate the molecular mechanism of CD36 deficiency. [Methods] Flow cytometry was performed in the southern Chinese population to detect the CD36 phenotype. Among them, 15 cases of CD36 type I deficiency, 15 cases of CD36 type Ⅱ deficiency, and 10 positive samples were selected. The TGS of the CD36 gene was performed and statistical analysis was conducted. [Results] 40 samples (including 15 cases of type I deficiency, 15 cases of type Ⅱ deficiency, and 10 positive samples) were subjected by TGS of CD36 full-length sequences (except part of intron1). A total of 180 polymorphic loci were identified. Among them, 13 kinds were in the coding region, the rest were in non-coding region, with most mutations located in regulatory regions such as the 5′-UTR and 3′-UTR. [Conclusion] The high polymorphism of CD36 non-coding regions, particularly in regulatory sequences, provides mechanistic insights into type Ⅱ CD36 deficiency.
Authors and Affiliations
Jing LIU, Xiuzhang XU, Haoqiang DING, Jing DENG, Yangkai CHEN, Wenjie XIA, Xin YE
Keywords
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- EP ID EP767947
- DOI 10.13303/j.cjbt.issn.1004-549x.2025.05.003
- Views 10
- Downloads 0
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