Preliminary Blood Compatibility Comparison of Silk Fibroin Dissolved by different Solvents
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2018, Vol 12, Issue 2
Abstract
Silk fibroin possesses good cytocompatibility and biodegradability, was a potential material for wound haemostasis. Ca2+ act as a coagulation factor in cascade pathway for thrombin generation, it can be chelated to silk fibroin molecule. We used three neutral salt solvents (CaCl2, Ca(NO3)2 and LiBr) to dissolve silk fibers and prepare regenerated silk fibroin materials, preliminarily explored blood compatibility. Results showed that the regenerated silk fibroin materials prepared by calcium salt dissolving could shorten thrombin time and plasma recalcification time, compared with non-calcium salt reagent LiBr.Haemorrhage still remains a leading cause of early death and infectious complications in trauma or combat wounds continue to challenge caregivers. Various materials (such as gelatine, fibrin glue, chitosan, collagen, and inorganic microporous materials) have been developed as haemostatic materials [1-5]. On market currently, gelatine and chitosan have attracted several clinically proven products for wound haemostasis. Collagen is also considered a promising biomaterial for wound haemostasis [1,5], but it do not have the ability to induce the rapid hemostasis required for surgical applications [6,7]. Chitosan, as a haemostatic material, is able to stop low pressure arterial bleeds, but is flimsy to maintain hemostasis after intravenous administration of fluids, leading to the risk of rebleeding [8]. After modification, the haemostatic efficiency of chitosan can be improved [9,10]. Collagen, gelatine and chitosan do not have physiological haemostatic functions. In the coagulation mechanism, Ca2+ is indispensable in cascade pathway for thrombin generation. On a wound, Ca2+ interacts with platelet surface phospholipids to activate thrombin, and then digest fibrinogen into fibrin, resulting in coagulation formation. So, calcium alginate has been paid more attention to the applications in embolization [11-13]. However, the cytocompatibility of calcium alginate is not satisfactory. Therefore, it is desired to develop a haemostatic materials with comprehensive ability, combining physical properties, good cytocompatibility and physiologically haemostatic properties. Silk fibroin is a natural animal protein, rich in sources, however, the developments on the haemostatic function of SF materials are lacking. Silk fibroin possesses good cytocompatibility and biodegradability, has been proved for cells adhere and proliferate [14-16], also has been considered as potential tissue engineering scaffolds and drug delivery carriers [16-21]. Interestingly, silk fibroin has a large number of functional groups, such as -COOH of glutamic acid or aspartic acid, -NH2 of lysine and -OH of serine or Tyrosine [22-24], which can interact with Ca2+ by electrostatic interaction and coordination bonds. In the study, we prepared silk fibroin porous haemostatic materials by different solvents, and investigated Hemolysis and clotting capability, expecting to expand the application of silk fibroin in haemostatic materials.
Authors and Affiliations
Ling zhang, Mengyao Ding, Guangzhou Song, Wei Tian, Helei Li, Honggen Yi, Yin Yin, Fenglin Dong, Jiannan Wang
Keywords
Related Articles
- EP ID EP586971
- DOI 10.26717/BJSTR.2018.12.002233
- Views 163
- Downloads 0
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