Preliminary investigation on optimum condition of to induce mutation for development new variability in opium poppy ([i]Papaver somniferum[/i] L.)
Journal Title: Rośliny Oleiste - Oilseed Crops - Year 2013, Vol 34, Issue 2
Abstract
The aim of the studies was to identify optimum method for inducing mutagenesis to obtain new of alkaloid variation in poppy.The study material consisted of breeding lines derived from recombinants of various forms of high-morphine poppy from the IHAR-PIB collection, and the high-morphine Lazur variety.The studies were carried out over three vegetation seasons. A total of 14 methods of mutagen treatment were employed, using varying concentrations of the chemical and ultrasounds, and different mutagen exposure times. Seeds were treated with ethyl methanesulphonate (EMS) and ultrasounds (US) in order to facilitate the penetration of mutagenic substances into the seeds. Mutagen concentrations were: 0.4; 0.6; 0.8; 1.0; 1.2; 1.4 and 1.6% EMS. Seeds of selected poppy genotypes were kept in the solution for three or four hours depending on mutagen concentration. The studies also involved investigation of the effect of ultrasound treatment on the increase in effectiveness of chemical mutagenesis in poppy seeds of the Lazur variety. The duration of seed exposure to ultrasound treatment was 5 or 25 minutes. Following decantation, the seeds were washed under running water and dried on tissue paper. The seeds that had been subjected to mutagen treatment and control lines were then propagated in selection nurseries. It was possible to achieve higher variation in the content of morphine, codeine or thebaine. Extended range of variation in alkaloid content in poppy capsules demonstrates that EMS is an efficient chemical mutagen suitable for the development of new genotypes.
Authors and Affiliations
Magdalena Walkowiak, Maria Ogrodowczyk
Keywords
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- EP ID EP59757
- DOI 10.5604/12338273.1101233
- Views 130
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