Preparation and Characteristic of Dextran-BSA Antibody and Establishment of it’s Elisa Immunoassay
Journal Title: International Journal of Food Science, Nutrition and Dietetics (IJFS) - Year 2014, Vol 3, Issue 6
Abstract
The enzyme linked immunosorbent assay (ELISA) is a potential tool for the determination of dextran. In this study, dextran–BSA antigens were prepared by Reductive amination method, and were confirmed by SDS-PAGE and free amino detection. The effects of coupled reaction conditions such as different oxidation degree of dextran, the reaction time were investigated and the immunity of the resulting dextranBSA neoglycoprotein antigens were evaluated through the interaction with standard dextran antibody. The immunogen was immunized with white rabbits to obtained polyclonal antibody respectively. A general and broad class-specific Elisa detection method was developed according to Elisa theory. The method was put to use for quantitative analysis of dextran in practical saccharose samples.
Authors and Affiliations
Xie Zhen-ming
Keywords
Related Articles
- EP ID EP200586
- DOI 10.19070/2326-3350-1400024
- Views 104
- Downloads 0
Polyphenol-rich fruits attenuate impaired endothelial function induced by glucose and free fatty acids in vitro in human endothelial cells
Elevated concentrations of plasma glucose and free fatty acids (FFA) are associated with impaired endothelial function increasing risk of cardiovascular diseases. Previous studies suggested that fruits rich in polyphenol...
Comparative Effects Of Processing On The Cyanide Content Of Manihot Esculenta , Glycine Max And Zea Mays
The effects of varying processing treatments on the cyanide content of Manihot Esculenta, Zea Mays and Glycine Max were determined using picrate kit method and the following mean concentrations in ppm were obtained: 0.10...
A Study on the Prevalence of Obesity in Type II Diabetes Mellitus among Female Adults Aged 20-50 Years Attending Abha and Khamis Mushayat Diabetic Centres in Saudi Arabia
Background: A sedentary lifestyle and prevalence of obesity are the key factors which lead to an increasing prevalence of Type II diabetes mellitus. There is strong evidence that modifiable risk factors such as obesity a...
Protective Effect on Lipids Profile of Lion’s Foot (Alchemilla Vulgaris) Leaves against Ccl4 Toxicity and its Fortified to Guava and Mango Pulp
Lion’s foot (Alchemilla vulgaris L.) which is widely known as lady’s mantle, bear’s foot or lion’s foot is traditionally used due to their tannin contents for the treatment of inflammations, diarrhea, wound healing and i...
Serum levels of Magnesium, Copper and Zinc in Young Couples
Metal ions are required for essential body functions but are toxic in excess. These have been examined critically as a potent factor in various human diseases including cardiovascular disease, cancer. This study was cond...