Preparation and Evaluation of Immunogenicity of Chitosan Nanoparticles Containing Tetanotoxin in Comparison with Freund\'s Adjuvant in Mouse Model for Improvement of Tetanus Vaccine after Subcutaneous Injection
Journal Title: مجله علمی- پژوهشی دانشگاه علوم پزشکی قم - Year 2017, Vol 11, Issue 2
Abstract
Background and Objectives: Tetanus is a deadly zoonotic infectious disease. For nearly a century, tetanus vaccine has been produced and used. This study was carried out with the aim of preparation and evaluation of immunogenicity of chitosan nanoparticles containing tetanotoxin in comparison with Freund's adjuvant in mouse model for improvement of tetanus vaccine after subcutaneous onjection. Methods: In this experimental study, chitosan nanoparticles synthesis containing tetanus toxoid was performed using ionic gelation method. The morphology of nanoparticles was investigated using SEM and AFM, then, the nanoparticles were subcutaneously injected into mice and two weeks after the end of injection, blood was taken. Antibody titer was measured by indirect ELISA. Data were analyzed using one-way one-way ANOVA and Tukey test at significance level of p<0.05. Results: High-load antigen (uniform spherical morphology and the mean size of 144), were encapsulated in chitosan nanoparticles. After serum analysis, IgG antibody titer was significantly different between the mice that received antigen as nanoparticle and the control group, but no significant difference was observed in the antigen with Freund’s adjuvant group. Conclusion: Based on the results of this study, tetanus toxoid injection using nanoproces is able to induce humoral immune response almost equal to Freund’s adjuvant and can be an appropriate alternative.
Authors and Affiliations
Majdedin Majdedin, Mojtaba Mojtaba, Jafar Jafar, Ebrahim Ebrahim, Yoosuf Yoosuf, Roohullah Roohullah, Morteza Morteza, Alimohammad Alimohammad
Keywords
Related Articles
- EP ID EP464803
- DOI -
- Views 50
- Downloads 0
Antibacterial Effect of Nano Polyamidoamine-G7 Dendrimer on Clostridium perfringens, Bacillus subtilis, Salmonella typhi, and Shigella dysenteriae in Aqueous Solution
Background and Objectives: A large number of studies has been conducted on applications of Polyamidoamine dendrimers, but their antibacterial activity has not been investigated extensively. The purpose of this study was...
The Effect of Training on Self-Care and Controlling Disease-Related Complications in Woman with Multiple Sclerosis: A Randomized Clinical Trial
Background and Objectives: Multiple sclerosis (MS) is a chronic disease of central nervous system, which causes a lot of complications and problems for patients and leads to their disability and dependency in life. The a...
تأثیر آنتی‌اکسیدانتی مرزه خوزستانی بر باروری و رشد جنین‌های حاصل از لقاح داخل آزمایشگاهی موش‌های سوری نر بالغ تحت درمان با نانیل‌فنل
زمینه و هدف: نونیل‌فنل نوعی از مواد شیمیایی محیطی است که مانند هورمون آندروژن عمل کرده و مانع از فعالیت هورمون‌ها، سبب ایجاد اختلالات سیستم تولیدمثلی و القای استرس اکسیداتیو می‌شود. در این مطالعه اثر محافظتی مرزه خوزستان...
The Lived Experiences of Patients Waiting for Coronary Artery Bypass Grafting: A Hermeneutic Phenomenological Study
Background and Objectives: Waiting for coronary artery bypass grafting is an unpleasant experience for patients and is associated with physical and psychological problems. The aim of this study was to recognize lived exp...
استفاده از آنتی‌ژن‌های وزن مولکولی پایین مایکوباکتریوم توبرکلوزیس به‌عنوان یک تست پوستی جدید جهت تشخیص اختصاصی سل
زمینه و هدف: سل یک بیماری عفونی است و در حدود یک‌سوم مردم دنیا به عفونت نهفته سل آلوده‌اند. عفونت سل فعال ممکن است در 10-5% از افراد مبتلا به عفونت نهفته ایجاد شود؛ بنابراین تشخیص عفونت نهفته در کنترل بیماری بسیار مهم اس...