Preparation and preliminary application of aquaporin 4 (AQP4) specific monoclonal antibody
Journal Title: Eye Science (Yanke Xuebao) - Year 2021, Vol 36, Issue 8
Abstract
[Objective:] To lay the foundation for clinical applications in the future, we prepare and identify the immunological properties of aquaporin 4 (AQP4) monoclonal antibodies. [Methods:] The dominant epitope polypeptides of AQP4 were synthesized and used to obtain the cell lines which secrete high tiler and high affinity monoclonal antibodies by immunizing BALB/c mouse cell fusion and screening of subclone. The large amount of monoclonal antibodies were obtained and used for practice via immunohistochemical staining and Enzyme-linked immunosorbent assay (ELISA). [Results:] Four hybridomas that can stably secret anti-AQP4 antibodies were obtained. ELISA, Western blot and Immunohistochemistry results suggested that the monoclonal antibodies specifically recognized human AQP4 protein. [Conclusion:] The anti-human AQP4 monoclonal antibody with high affinity and specificity was successfully generated, which thereby provides the foundation for the preparation of small molecule antibodies or humanized antibodies, and then lays a theoretical and technical basis for the treatment of neuromyelitis optica (NMO).
Authors and Affiliations
Di ZHAO, Yuli ZHANG, Huiying KANG, Qijiang CAO
Keywords
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- EP ID EP763550
- DOI 10.3978/j.issn.1000-4432.2021.07.30
- Views 17
- Downloads 0
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