Preparation and preliminary application of aquaporin 4 (AQP4) specific monoclonal antibody
Journal Title: Eye Science (Yanke Xuebao) - Year 2021, Vol 36, Issue 8
Abstract
[Objective:] To lay the foundation for clinical applications in the future, we prepare and identify the immunological properties of aquaporin 4 (AQP4) monoclonal antibodies. [Methods:] The dominant epitope polypeptides of AQP4 were synthesized and used to obtain the cell lines which secrete high tiler and high affinity monoclonal antibodies by immunizing BALB/c mouse cell fusion and screening of subclone. The large amount of monoclonal antibodies were obtained and used for practice via immunohistochemical staining and Enzyme-linked immunosorbent assay (ELISA). [Results:] Four hybridomas that can stably secret anti-AQP4 antibodies were obtained. ELISA, Western blot and Immunohistochemistry results suggested that the monoclonal antibodies specifically recognized human AQP4 protein. [Conclusion:] The anti-human AQP4 monoclonal antibody with high affinity and specificity was successfully generated, which thereby provides the foundation for the preparation of small molecule antibodies or humanized antibodies, and then lays a theoretical and technical basis for the treatment of neuromyelitis optica (NMO).
Authors and Affiliations
Di ZHAO, Yuli ZHANG, Huiying KANG, Qijiang CAO
Observational study of a corneal dystrophy family with confocal microscope
[Objective:] To observe and study the corneal in patients of family with granular corneal dystrophy by confocal laser scanning microscopy. [Methods:] The corneas of 4 patients (6 eyes) with granular corneal dystrophy wer...
Clinical application of esmketamine hydrochloride combined with propofol in curettage of eyelid tumors in children
[Objective:] To investigate the anesthetic effect and to evaluate the efficacy and safety of combination application of esketamine and propofol in curettage of eyelid tumors of children. [Methods:] This study selected 70...
Application of a total quality management model based on the DingTalk platform in ophthalmology education
[Objective:] To combine the characteristics of standardized training for ophthalmic resident physicians, establishes a Total Quality Management (TQM) model based on the DingTalk platform, and explored an efficient ophtha...
Boston keratoprosthesis type I: application and development
The corneal transplantation is an effective option for visually impaired patients with keratopathy to restore vision function. However, the success rate of allograft keratoplasty is still very low for those patients with...
Correlation between work-family balance and work efficiency of standardized trainees in ophthalmology hospitals
[Objective:] To explore the correlation between work-family balance and work efficiency of ophthalmology standardized trainees in ophthalmology hospitals. [Methods:] A questionnaire survey was conducted among 140 ophthal...