Preparation and preliminary application of aquaporin 4 (AQP4) specific monoclonal antibody

Journal Title: Eye Science (Yanke Xuebao) - Year 2021, Vol 36, Issue 8

Abstract

[Objective:] To lay the foundation for clinical applications in the future, we prepare and identify the immunological properties of aquaporin 4 (AQP4) monoclonal antibodies. [Methods:] The dominant epitope polypeptides of AQP4 were synthesized and used to obtain the cell lines which secrete high tiler and high affinity monoclonal antibodies by immunizing BALB/c mouse cell fusion and screening of subclone. The large amount of monoclonal antibodies were obtained and used for practice via immunohistochemical staining and Enzyme-linked immunosorbent assay (ELISA). [Results:] Four hybridomas that can stably secret anti-AQP4 antibodies were obtained. ELISA, Western blot and Immunohistochemistry results suggested that the monoclonal antibodies specifically recognized human AQP4 protein. [Conclusion:] The anti-human AQP4 monoclonal antibody with high affinity and specificity was successfully generated, which thereby provides the foundation for the preparation of small molecule antibodies or humanized antibodies, and then lays a theoretical and technical basis for the treatment of neuromyelitis optica (NMO).

Authors and Affiliations

Di ZHAO, Yuli ZHANG, Huiying KANG, Qijiang CAO

Keywords

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  • EP ID EP763550
  • DOI 10.3978/j.issn.1000-4432.2021.07.30
  • Views 16
  • Downloads 0

How To Cite

Di ZHAO, Yuli ZHANG, Huiying KANG, Qijiang CAO (2021). Preparation and preliminary application of aquaporin 4 (AQP4) specific monoclonal antibody. Eye Science (Yanke Xuebao), 36(8), -. https://europub.co.uk/articles/-A-763550