Preparation of Eco-friendly Antibacterial Silver Nanoparticles from Leaf Extract of Ficus Benjamina
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2018, Vol 9, Issue 4
Abstract
Purpose: Present study was conducted to prepare simple, low cost and quick in action silver nanoparticles (Ag NPs) using silver nitrate and eco-friendly biological material (leaves of Ficus benjamina), for four pathogenic bacteria. Methods: The leaf extract of Ficus benjamina was prepared by soaking 10 g washed leaves in 90 mL of distilled water and were boiled at 90 °C for 20 minutes; filtrate was stored at 4°C. Silver nanoparticles were prepared by adding 10 mL of filtrate in 50 mL of 1 mM silver nitrate solution, sonicated at 60°C for 60 minutes. A change in the color from yellow to brown indicated the formation of Ag NPs. Ag NPs were characterized by SEM, XRD and FTIR techniques and their antibacterial activity was estimated by disc diffusion method. Results: The solution changed from a light yellowish to dark brown, caused by a reduction in the number of silver ions, confirming the synthesis of Ag NPs. The XRD spectrum revealed the crystalline Ag NPs were of 13.2 nm size. The FTIR and SEM images showed that the Ag NPs were fairly spherical. The disc-diffusion showed that prepared Ag NPs strongly inhibited the pathogenic activities of Bacillus subtilis, Pasteurella multocida, Staphylococcus aureus and Enterobacter aerogenes. The inhibition zone ranged from 7 mm to 13 mm. Conclusion: Present findings indicated that the green synthesis of Ag NPs has many advantages over other chemical procedures, offering enhanced safety, eco-friendlier and more superior in results.Nanotechnology is an emerging field science having vast scope in research and development with the aim of production and implications of new materials having nanoscale dimension [1, 2]. Nanoparticles are atoms with size up to 100 nm [3] showing fascinating properties established on particular features such as size, utility and morphology. Characterization is performed to access different properties of the nanoparticles [4, 5]. Literature suggested a variety of nanoparticles utilization for different purposes like treatment of diseases and disorders but silver nano particles showed dominant antimicrobial activity [1,6]. They also have anti-inflammatory and anti-viral activity [7]. Silver is also used in silver-based ointments to cure wounds, burns and medical related strategies that are associated with polymers saturated with silver [8]. Utilization of silver (Ag) nanoparticles, particularly prepared with medicinal plants is a novel approach to treat microbial pathogen, furthermore, secondary metabolites in plant extracts play an important role in the synthesis of nano silver particles having strong antibacterial potential [9].
Authors and Affiliations
Sara Zafar, Asma Ashraf, M Yasin Ashraf, Farkhanda Asad, Shagufta Perveen, M Amir Zafar, Andleeb Shahzadi4
Keywords
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- EP ID EP591789
- DOI 10.26717/BJSTR.2018.09.001829
- Views 166
- Downloads 0
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