Preparation of Plasminogen by Affinity Chromatography
Journal Title: Iranian Journal of Blood and Cancer - Year 2014, Vol 6, Issue 4
Abstract
Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the starting material. By increasing its concentration of ethanol from 0% to 8% in pH 7.2 at -3○C the centrifugation was carried out and the supernatant I was separated from precipitate I. Then supernatant fraction I was used for separation of human plasminogen from plasma and further purification was carried out using affinity chromatography using affinity gel lysine Sepharose. Results: In our study we could obtain plasminogen with the concentration of 0.5%. In normal human plasma the concentration of plasminogen is about 200mg/l. Comparing the result with the concentration of plasminogen in normal human plasma shows about 25 folds higher concentration. Conclusion: Using affinity chromatography method a 25-fold increase in plasminogen concentration was achieved which shows this method is a promising method for the preparation of plasminogen from human plasma.
Authors and Affiliations
K Mousavi Hosseini, M. Nikougoftar Zarif
Keywords
Related Articles
- EP ID EP363422
- DOI -
- Views 73
- Downloads 0
Short-term Chelating Efficacy of Deferoxamine in Iron Overloaded Rat Hepatocytes
Abstract Background: Iron overload is a clinical consequence of repeated blood transfusions and causes significant organ damage, morbidity, and mortality in the absence of proper treatment. The primary targets of Iron ch...
Pediatric Non Hodgkin’s Lymphomas
Pediatric non-Hodgkin lymphoma (NHL) is a diverse collection of diseases, and results from malignant proliferation of lymphoid cells and immune system. NHL involves throughout the body, but bone and primary central nervo...
Multiple Myeloma with HBs Antigenemia Presented with Hoarseness and Systemic Amyloidosis
This article has no abstract.
Prevalence of Diabetes Mellitus in Patients with Transfusion Dependent β Thalassemia
Introduction: Thalassemia is common in Iran. Appropriate therapy for this disease includes a regular blood transfusion and chelation therapy. However, patients will inevitably confront with side effects, particularly iro...
Changing Pattern of Histone H3 Methylation following treatment of erythroid progenitors derived from cord blood CD133+ cells with sodium butyrate and thalidomide
Background: Human β-like globin genes regulaon during development from embyonic to adult stage results in generaon of different types of hemoglobin with different funcons. As β-thalassemia and sickle cell disease are...