Prevalence and antibiotic profile of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in beef and buffalo
Journal Title: Food Research - Year 2019, Vol 3, Issue 1
Abstract
Shiga-toxin producing Escherichia coli bacteria are well known to be the pathogenic bacteria that cause traveler diarrhea. E. coli O157: H7 from the group of Shiga-toxin producing E. coli cause even severe infection which can lead to fatality for humans. In this study, local beef and Indian buffalo were selected to determine the presence of Shiga-toxin producing E. coli and E. coli O157: H7 using Most Probable Number-Polymerase Chain Reaction (MPN-PCR) method. Among 108 samples, eight (7.41%) samples from local beef and Indian buffalo were detected a positive on E. coli O157: H7 while thirteen (12.04%) samples were detected positive for Shiga-toxin producing E. coli gene. Out of 108 samples, eleven isolates of E. coli O157: H7 were successfully isolated in order to carry out the antibiotic susceptibility test. Shiga-toxin producing E. coli isolates were found susceptible to ceftazidime (100%), moxifloxacin (83.33%), sulphamethoxazole (66.67%), ampicillin (50%), amoxycillin (50%), ciprofloxacin (50%), erythromycin (33.33%) and penicillin G (33.33%). E. coli O157: H7 isolates were susceptible toward erythromycin (100%), ceftazidime (100%), ciprofloxacin (100%) and moxifloxacin (100%), sulphamethoxazole (60%), ampicillin (20%), amoxycillin (20%), and penicillin G (0%). The safety of both local beef and Indian buffalo was challenged by the presence of both Shiga-toxin producing E. coli and E. coli O157: H7. Better and safer ways of removing the pathogen from local beef and Indian buffalo should be researched more deeply.
Authors and Affiliations
S. Y. Kwan, W. S. Chang, Y. Y. Loo, Y. Nordin, C. W. Tan, C. H. Kuan, C. Y. New, E. Lee, Nor-Khaizura M. A. R. , Son Radu
Keywords
Related Articles
- EP ID EP491225
- DOI 10.26656/fr.2017.3(1).218
- Views 181
- Downloads 0
Detection of Vibrio cholerae in street food (satar and otak-otak) by Loop-Mediated Isothermal Amplification (LAMP), multiplex Polymerase Chain Reaction (mPCR) and plating methods
This goal of this study was to investigate the presence of Vibrio cholerae in street food, namely satar and otak-otak, using Loop-Mediated Isothermal Amplification (LAMP), multiplex Polymerase Chain Reaction (mPCR) and c...
Effect of food additives on the quality of white shrimp (Litopenaeus vannamei)
The effects of three additives: sodium tripolyphosphate (STPP), sodium bicarbonate (NaHCO3) and microbial transglutaminase (MTGase) with the addition of 2% sodium chloride (NaCl) at different concentrations (2, 5 and 8%)...
Ambit determination method in estimating rice plant population density
Rice plant population density is a key indicator in determining the crop setting and fertilizer application rate. It is therefore essential that the population density is monitored to ensure that a correct crop managemen...
Evaluation of antioxidant capacity of Aidia borneensis leaf infusion, an endemic plant in Brunei Darussalam
We investigated the total antioxidant capacity of Aidia borneensis leaf infusion, a Bornean endemic plant, which is traditionally consumed as a home-remedy beverage in Brunei Darussalam. The antioxidant capacity of the i...
New coating material for producing virgin coconut oil (VCO) microcapsules
The aim of this work was to investigate the microencapsulation efficiency (MEE) of different grades of broken rice (RB) and breadfruit (BB)-based maltodextrin as a coating material, using virgin coconut oil (VCO) as a mo...