Prevalence and antibiotic profile of Shiga-toxin producing Escherichia coli and Escherichia coli O157: H7 in beef and buffalo
Journal Title: Food Research - Year 2019, Vol 3, Issue 1
Abstract
Shiga-toxin producing Escherichia coli bacteria are well known to be the pathogenic bacteria that cause traveler diarrhea. E. coli O157: H7 from the group of Shiga-toxin producing E. coli cause even severe infection which can lead to fatality for humans. In this study, local beef and Indian buffalo were selected to determine the presence of Shiga-toxin producing E. coli and E. coli O157: H7 using Most Probable Number-Polymerase Chain Reaction (MPN-PCR) method. Among 108 samples, eight (7.41%) samples from local beef and Indian buffalo were detected a positive on E. coli O157: H7 while thirteen (12.04%) samples were detected positive for Shiga-toxin producing E. coli gene. Out of 108 samples, eleven isolates of E. coli O157: H7 were successfully isolated in order to carry out the antibiotic susceptibility test. Shiga-toxin producing E. coli isolates were found susceptible to ceftazidime (100%), moxifloxacin (83.33%), sulphamethoxazole (66.67%), ampicillin (50%), amoxycillin (50%), ciprofloxacin (50%), erythromycin (33.33%) and penicillin G (33.33%). E. coli O157: H7 isolates were susceptible toward erythromycin (100%), ceftazidime (100%), ciprofloxacin (100%) and moxifloxacin (100%), sulphamethoxazole (60%), ampicillin (20%), amoxycillin (20%), and penicillin G (0%). The safety of both local beef and Indian buffalo was challenged by the presence of both Shiga-toxin producing E. coli and E. coli O157: H7. Better and safer ways of removing the pathogen from local beef and Indian buffalo should be researched more deeply.
Authors and Affiliations
S. Y. Kwan, W. S. Chang, Y. Y. Loo, Y. Nordin, C. W. Tan, C. H. Kuan, C. Y. New, E. Lee, Nor-Khaizura M. A. R. , Son Radu
Keywords
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- EP ID EP491225
- DOI 10.26656/fr.2017.3(1).218
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