Prevalence and Antibiotic Susceptibility Pattern of Methicillin Resistant Staphylococcus aureus in Tertiary Care Hospitals
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 3
Abstract
Aims: Methicillin resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in hospitals with hospital based outbreaks world-wide. The present study was carried out to estimate the prevalence of MRSA isolates in clinical specimens and to investigate the sensitivity pattern of resistant isolates against various antibiotics used for treating hospitalized patients. Study Design: The percentage of MRSA was defined as the number of S. aureus isolates resistant to methicillin divided by the total number of S. aureus isolates for which methicillin susceptibility test results were reported. Place and Duration of the Study: The study was conducted during January 2012 to December 2012 at Asian Hospital (tertiary care hospital), Faridabad, Delhi. Methodology: A total of 107 isolates of S. aureus were identified by standard laboratory procedures including catalase test, slide and tube coagulase tests, β- haemolysis on blood agar and growth on mannitol salt agar. Subsequently, antibiotic sensitivity pattern of S. aureus were determined by automated antibiotic susceptibility testing system Vitek 2 compact identification and susceptibility testing (ID/AST) system by using AST-GP-67 card. Result: 23 isolates (21.4%) were identified as MRSA and the remaining 84 (78.6%) were methicillin sensitive S. aureus (MSSA). All MRSA isolates were found to be resistant to penicillin, oxacillin, ciprofloxacin and levofloxacin. The antibiotic resistance pattern of these isolates were high against trimethoprim-sulfamethoxazole (91.3%), erythromycin (47.8%), gentamicin (43.5%), moxifloxacin (42.9%), and less resistance rate was observed against tetracycline (30.4%) rifampicin (13.6%) and clindamycin (4.8%). However, all isolates of Staphylococcus were sensitive to vancomycin, linezolid, nitrofurantoin, quinpristin/daflopristin. Conclusion: Findings presented in this study indicated a high level of resistance to widely used therapeutic agents. An appropriate knowledge on the current antibiotic susceptibility pattern of MRSA is essential for appropriate therapeutic scenario.
Authors and Affiliations
A. Varma, N. Kaur, R. Prasad
Keywords
Related Articles
- EP ID EP237214
- DOI 10.9734/BBJ/2014/4245
- Views 88
- Downloads 0
Screening and Media Optimization for Enhancing L-asparaginase Production, an Anticancer Agent, from Different Filamentous Fungi in Solid State Fermentation
Aim: The aim of present study was to screen new potent fungal isolates and microorganisms possessing extracellular L-asparaginase production capacity. In addition, optimization of cultural and environmental conditions re...
Development of an Efficient Plant Regeneration System of Field Mustard (Brassica campestris)
Aims: The present study was conducted with a view to develop an efficient protocol for high frequency plant regeneration of Brassica campestris for further crop improvement program by biotechnological manipulation and to...
Assessment of Roof Harvested Rainwater in Parts of Anambra State for Environmental Pollution Monitoring
Questions have been raised about the quality of roof harvested rainwater which has been shown to be temporally and spatially variable and at times not in compliance with drinking water guidelines. This study is therefore...
Ethnobotanical, Nutritional, Phytochemical and Antimicrobial Studies of Garcinia xanthochymus Fruit Extracts
Aim: An attempt has been made to document the ethnobotanical values of the fruits of G. xanthochymus in and around the Similipal Biosphere Reserve (SBR) forest and evaluate the nutritional, phytoc...
In vitro Antibacterial Activity of Snake Venom, Naja naja from Bangladesh
Aim: Snake venom is a source of antimicrobial peptides. Composition as well as activities of venom may vary based on geographic region and notably the antimicrobial activity of the venom of Naja naja from Bangladesh has...