Prevalence and Molecular characterization of the Human Rotavirus strains detected in children suffering from acute gastroenteritis at Wardha
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH IN MEDICAL SCIENCES - Year 2016, Vol 2, Issue 2
Abstract
Introduction: Human rotavirus infection remains a significant clinical problem through the world, infecting almost every child below 5 year age. Material and Method: Clinical presentations of acute gastroenteritis cases during the study period were analyzed. Rotavirus diarrhea was confirmed with ELISA. ELISA rotavirus positive samples were tested for molecular characterization (G & P typing), done by conventional polymerase chain reaction. Results: Between July 2010 and May 2013, among the 341 enrolled children below 5 years, a total of 40 were positive for rotavirus by the ELISA test. The prevalence of rotavirus diarrhoea 11.7%.The most common genotype wereG1 (57.5) followed by G2 (20%), G3 (10%), G4 (2.5%) and G9 (2.5%).The P type identified were P[4] ( 27.5%), P[8](47.5%), P [6](20%). The most common age of rotavirus diarrhea was 12-17 months. The predominant symptoms were watery diarrhea, fever, and vomiting. Conclusion: The prevalence of rotavirus diarrhea among children less than 5 years of age was 11.7%. and circulating stains of rotavirus was G1, G2,G3,G4 & G9.
Authors and Affiliations
Nilesh Shyam Chavan1* Dr. Nilima Tankhiwale2
Keywords
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