Prevalence, antibiotic resistance pattern and identification of extended spectrum beta-lactamase producing Klebsiella pneumoniae by phenotypic and genotypic method from various clinical samples
Journal Title: Indian Journal of Pharmacy and Pharmacology - Year 2019, Vol 5, Issue 4
Abstract
The present study is about ESBL producing Klebsiella pneumoniae which is diagnosed by phenotypic and genotypic methods A total of 5055 properly collected well labelled samples received in the Microbiology laboratory were processed following standard protocol Total 5055 samples 805 160 Klebsiella pneumoniae were isolated 2263415 664 urine 218649 336 wound 127342 371 blood 215604 356 pus and 1845400 sputum samples tested positive for Klebsiella pneumoniae The occurrence of ESBL producers among Klebsiella pneumoniae in the current study is 293805 364 Highly resistant drugs for Klebsiella pneumoniae ESBL isolates were Ampicillin 100 Piperacillin 100 Amoxycillin clavulanic acid 662 Non ESBL producing isolates were less resistant to the same antibiotics Out of 805 isolated 285 354 and 293 363 were identified as ESBL producers by double disc synergy test and phenotypic confirmatory test respectively Samples identified as ESBL producers were confirmed by E test Detection of gene among these isolates single bla gene could be detected in 59 201 two genes in 71 242 and three genes in 156 532 isolates blaCTXM15 was the only gene found in 8 4 of the Klebsiella pneumoniae isolates tested whereas blaSHV alone was found in 42 143 isolates Among the combination of two bla genes the combination of blaSHV and blaCTXM15 was found in 54 184 isolates This study establishes that considerable genetic diversity and variations in the distribution of ESBL producers and their bla genes exist in the clinical isolates of K pneumoniae across KarnatakaKeywords ESBL Klebsiella pneumoniae CLSI guidelines Gene
Authors and Affiliations
Bilal Ahmad Mir, Shireen Rana, S B. Siddesh
Keywords
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- EP ID EP478574
- DOI 10.18231/2393-9087.2018.0042
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