PREVALENCE OF AMPC β-LACTAMASE GENES AMONG CLINICAL ISOLATES OF ENTEROBACTER SPP. FROM SOME IRAQI HOSPITALS.
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 4
Abstract
Background: Resistance to broad spectrum β-lactams mediated ESBLs and AmpC β-lactamase enzymes is a rising problem worldwide. Determination of their prevalence is essential to the creation of an effective antibiotics policy and hospital infection control measures. Method: A total of 35 clinical isolates of Enterobacter spp. that were collected from different clinical specimens during the period between October 2015 to January 2016 were studied. Antibiogram profile was done by disc diffusion test (DDT), along with recommended tests for phenotypic detection of ESBLs and AmpC production. Isolates were also screened for their possessions of AmpC β-lactamase-encoding genes by using polymerase chain reaction (PCR) technique. Results: Antibiogram revealed that most Enterobacter spp. isolates were multi-drug resistant with a high resistance to cephalosporins; cephalexin(100%), cefazolin and cefotaxime(94.28%), while Susceptibility to imipenem. was highest(20%) followed by amikacin, pipracilli/tazobactam (22.85%).The phenotypic detection of ESBL and AmpC β-lactamases revealed that 25(71.42%), 29(82.85%) were ESBLs and AmpC β-lactamase producers respectively, in addition to 22(62.85%) of isolates were gave positive results for both ESBL and AmpC β-lactamases production. Detection of AmpC encoding genes revealed that a total of 28(96.55%) of 29 Enterobacter spp. were possessed AmpC genes that encoding for AmpC β-lactamases, with predominance of blaCIT, blaEBC and blaDHA genes, 16(57.14%), 11(39.28%), 8(28.57%) respectively. Conclusions: Phenotypic and genotypic methods for detection of AmpC-β-lactamese-producing Enterobacter spp. revealed that most of these isolates were AmpC β-lactamase producers;29(82.85%) and 28(96.55%) respectively, with predominance of CIT 16(45.71%) followed by 10(28.57%) , 8(22.85%) for EBC and DHA respectively. furthermore, 22(62.85%) of isolates were gave positive results for both ESBL and AmpC β-lactamases production.
Authors and Affiliations
Dr. Saad L. Hamed
Keywords
Related Articles
- EP ID EP628189
- DOI -
- Views 169
- Downloads 0
THE DIAGNOSTIC VALUE OF “CENTOR CRITERIA” IN CHILDREN WITH GROUP A BETA HEMOLYTIC STREPTOCOCCUS TONSILLOPHARYNGITIS
Background: Centor criteria (fever >38.5°C, swollen, tender anterior cervical lymph nodes, tonsillar exudate and absence of cough) include a scoring system used to assess the probability of group A β hemolytic Streptococ...
EVALUATION OF RESPONSE OF IMMUNE PARAMETERS IN HIV SUBJECTS ON ANTIRETROVIRAL THERAPY IN PORT HARCOURT
This cross sectional study was aimed at evaluating the response of immune parameters in HIV positive subjects on antiretroviral therapy in Port Harcourt, Rivers State, Nigeria. A total of three hundred subjects were invo...
SEQUENCE ALIGNMENT
A sequence alignment is a way of arranging the sequences of DNA, RNA, or protein to identify regions of similarity that may be a consequence of functional, structural, or evolutionary relationships between the sequences....
NANOMEDICINE: APPLICATION OF NANOTECHNOLOGY
This paper highlights the applications of nanotechnology in medicine termed “Nanomedicine”. Advances in nanomedicine centre on diagnosis (invitro and invivo), pharmaceuticals and therapeutics. Nanobiotechnology utilizing...
ASEAN – QUALITY REQUIREMENTS FOR GENERIC FILLING
ASEANrequires Process Validation report on three consecutive production batches which should include: Summary Introduction Batches used for validation Manufacturing equipment Critical process steps and parameters...